QUALITY CONTROL OF PYRAZINAMIDE IN FORMULATION USING MICELLAR LIQUID CHROMATOGRAPHY
AbstractPyrazinamide is an important anti-tubercular drug which shortens the course of TB chemotherapy. A direct injection liquid chromatographic method is developed for the determination of pyrazinamide in pharmaceutical formulation. The method includes a micellar mobile phase containing 0.15M sodium dodecyl sulphate and 1% butanol (v/v) buffered at pH3, a Princeton SPHER-100 C18 column (250mm × 4.6mm, 5µm particle size) and UV detection was set at 269nm. The Micellar Liquid Chromatography (MLC) method is rapid, precise, sensitive, and robust. In this method pharmaceutical samples were directly injected to the column without pre- treatment step. Under all these conditions, method has very short analysis time of 3.2 min, linearity (r > 0.998), limit of detection and limit of quantification is 1.4, 36.5 ng/ml respectively; Intra – and inter-day precision (RSD%) were 1.5, overall recovery in pharmaceutical formulation is 99.4%, 69.5%, 81.25%, 87.9%. The method is suitable for routine quality control analysis. This chromatographic techniques, MLC has the advantage of avoiding sample extraction step from matrices, thus reduces the time of analysis.
Article Information
16
4637-4644
417
1186
English
IJPSR
P. Mishra, A. Durgbanshi*, R. P. Pawar, G. Sharma and P. Biswas
Department of Chemistry, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, Madhya Pradesh, India.
abhiasha126@gmail.com
16 March, 2017
22 May, 2017
27 May, 2017
10.13040/IJPSR.0975-8232.8(11).4637-44
01 November, 2017
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