QUANTIFICATION OF DIOSGENIN IN EXTRACTS AND FORMULATIONS CONTAINING SOLANUM NIGRUM
AbstractA reversed phase high performance liquid chromatography coupled with diode array detector (HPLC–DAD) was developed and validated to quantitate diosgenin in berries of Solanum nigrum using C18 analytical column as stationary phase and acetonitrile: water (92: 08, v/v) as mobile phase. The flow rate of mobile phase was kept at 1.0 ml/min. Column oven temperature was set at 25 0C. The method was found to be linear over the range 1.0-60 µg/ml. The limit of detection and limit of quantitation of the developed method were found to be 0.33μg/ml and 1.0 μg/ml, respectively. The recoveries for the spiked samples ranged 96.85 – 103.68 %. The RSD values for intraday and interday precision studies of the method were all less than 1.11 %. The proposed method was found to be robust in terms of % w/w of diosgenin. The developed and validated method was applied for quantitative determination of diosgenin in extracts and formulations containing Solanum nigrum.
Article Information
20
676-681
395KB
1990
English
Ijpsr
Sonal Desai , Pratima Tatke * and S. Y. Gabhe
Professor of Pharm.Chem. C. U. Shah College Of Pharmacy, S. N. D. T. Women’s University, Sir Vithaldas Vidhyavihar, Juhu Road, Santacruz (W), Mumbai-400 049, India.
patatke@gmail.com
20 June, 2014
16 September, 2014
18 October, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.6(2).676-81
01 February, 2015
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