QUANTIFICATION OF LINEZOLID AND CEFUROXIME AXETIL BY CHEMOMETRICS ASSISTED AND RP-HPLC METHODS: DEVELOPMENT AND VALIDATION
AbstractThis paper describes simple, sensitive, accurate, rapid, precise and economical Chemometrics assisted and RP-HPLC methods for simultaneous estimation of Linezolid(LNZ) and Cefuroxime axetil(CEF) in bulk and tablet. Chemometrics offers a useful approach for an estimation of combined dosage form. The measurement carried out at wavelength 240 to 290nm with interval of 4nm for Linezolid and Cefuroxime axetil respectively. Chemometrics method were found to be linear (R2=0.999) for all four methods CLS, ILS, PLS and PCR for LNZ and CEF respectively. HPLC method by using mobile phase Acetonitrile: water 65:35 with Thermo Synchronies C-18 (250mm x 4.6mm, 5μm) flow rate 0.8ml/min and detection wavelength 267nm. Retention time was found to be 3.573 min for LNZ and 4.273 min for CEF. HPLC method was found to be linear (R2=0.999) for 6-30µg/ml for LNZ and 5-25µg/ml for CEF respectively. The applied methods Chemometrics and RP-HPLC were found to be successful for simultaneous estimation of LNZ and CEF in marketed dosage form
Article Information
34
3028-38
698
1462
English
IJPSR
Bhumika N Prajapati and Rajashree C. Mashru*
Department of Pharmacy, Shree G.H. Patel Pharmacy building, The Maharaja Sayajirao University of Baroda, Fatehgunj, Vadodara, Gujarat, India
rajshreemashru@yahoo.com
19 February, 2016
17 March, 2016
04 May, 2016
10.13040/IJPSR.0975-8232.7(7).3028-38
01 July 2016
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