QUANTITATIVE DETERMINATION OF PIPERACILLIN BY IODOMETRIC METHOD USING POTASSIUM PEROXOMONOSULFATE

Kinetics and stoichiometry S-oxidation reaction of sodium piperacillin by means of potassium hydrogen peroxomonosulfate in aqueous solutions using iodometric titration were studied. Zopercin^® – powder in vials for piperacillin injection solution in the combined form with tazobactam (piperacillin 4.0 g and tazobactam 0.5 g) was used for analysis. A new iodometric method for quantitative determination of sodium piperacillin in Zopercin^® preparation using potassium hydrogen peroxomonosulfate (KHSO₅) as analytical reagent was proposed. Peroxomonosulfate acid as triple potassium salt 2KНSO₅∙KНSO₄∙K₂SO₄ (Oxone^®) of “extra pure” qualification was used as oxidant. At pH 2-4 for 1 mole of penicillin, 1 mole of KHSO₅ is consumed; the quantitative interaction is achieved within a time of more than 1 minute (observation time). The results were obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species at various concentrations. RSD = 1.15 %, δ = (+0.2) %. It can be seen that piperacillin can be determined successively with good accuracy and reproducibility. The new procedure was developed and ability of quantitative determination of penicillin in pharmaceutical preparation Zopercin^® by iodometric method using potassium hydrogen peroxomonosulfate (KHSO₅) as analytical reagent was shown.