QUANTITATIVE DETERMINATION OF PIPERACILLIN BY IODOMETRIC METHOD USING POTASSIUM PEROXOMONOSULFATEAbstract
Kinetics and stoichiometry S-oxidation reaction of sodium piperacillin by means of potassium hydrogen peroxomonosulfate in aqueous solutions using iodometric titration were studied. Zopercin® – powder in vials for piperacillin injection solution in the combined form with tazobactam (piperacillin 4.0 g and tazobactam 0.5 g) was used for analysis. A new iodometric method for quantitative determination of sodium piperacillin in Zopercin® preparation using potassium hydrogen peroxomonosulfate (KHSO5) as analytical reagent was proposed. Peroxomonosulfate acid as triple potassium salt 2KНSO5∙KНSO4∙K2SO4 (Oxone®) of “extra pure” qualification was used as oxidant. At pH 2-4 for 1 mole of penicillin, 1 mole of KHSO5 is consumed; the quantitative interaction is achieved within a time of more than 1 minute (observation time). The results were obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species at various concentrations. RSD = 1.15 %, δ = (+0.2) %. It can be seen that piperacillin can be determined successively with good accuracy and reproducibility. The new procedure was developed and ability of quantitative determination of penicillin in pharmaceutical preparation Zopercin® by iodometric method using potassium hydrogen peroxomonosulfate (KHSO5) as analytical reagent was shown.
S. P. Karpova *, M. Y. Blazheyevskiy, O. O. Mozgova, Y. Y. Serdiukova, M. M. Ivashura and I. Y. Petukhova
Department of Physical and Colloid Chemistry, National University of Pharmacy, Pushkinska Street, Kharkiv, Ukraine.
23 October, 2017
20 December, 2017
25 December, 2017
01 July, 2018