DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC AREA UNDER CURVE METHOD QUANTITATIVE ESTIMATION OF PIPERACILLIN

The kinetic of conjugated reactions of S-oxidation and perhydrolysis of Piperacillin (Pip) with potassium hydrogen peroxo-monosulfate in alkaline medium is studied by the increase of forming product light absorbance. A scheme of peroxoacid oxidation and perhydrolysis conjugated reactions of Piperacillin by means of hydrogen peroxomonosulfate is proposed. The procedure of the quantitative analysis of Piperacillin in the combined with Tazobactam pharmaceutical preparation powder of Zopercin^® (Piperacillin 4.0 g and Tazobactam 0.5 g) by spectrophotometric – kinetic method is elaborated using triple potassium Caro’s salt solution as a reagent. Peroxomonosulfate acid as triple potassium salt 2КНSO₅∙КНSO₄∙ K₂SO₄ (Oxone^®) of “extra pure” qualification was used as oxidant. At pH 2-4 for 1 mole of Penicillin, 1 mole of KHSO₅ is consumed; the quantitative interaction is achieved within a time of more than 1 min (observation time). The method on initial rates (tangent method) was used to collect kinetic data (usually at 280 nm) by following the appearance product of perhydrolysis reaction of Piperacillin. A solution of sodium hydroxide was thermostated in the cell compartment, and then mixtures of solutions of Piperacillin / Tazobactam with solutions Caro’s acid (time incubation of 1 min) were added to the cell. The resulting solution was mixed thoroughly and put into the spectrophotometer. The precision of rate determination was usually ± 1-3%. The results were obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species at various concentrations. RSD = 1.4%. The obtained results have good agreement with the Parmacopoeia one δ = 1.0%.