QUANTITATIVE SIMULTANEOUS RP-HPLC ESTIMATION OF GALLIC ACID, QUERCETIN AND GLYCYRRHIZIN IN THE METHANOLIC EXTRACTS OF ABRUS PRECATORIUS AND CORDIA WALLICHI LEAVES

Background: Important mistletoe species include Abrus precatorius (Linn.) and Cordia wallichi (Don). According to reports, the leaves of Abrus precatorius (L.) have neuromuscular, antiepileptic, anti-diabetic, and many other properties. Leaves are traditionally administered to cuts, swellings, and mouth ulcers as a nerve tonic. Less studies have been recorded on the species of wallichi, compared toother species of the genus. According to reports, both plants’ leaves include alkaloids, flavonoids, tannins, terpenoids, saponins, steroids, and glycyrrhizin (Abrus). Objective: A simple, accurate and reproducible RP-HPLC method has been developed for the simultaneous quantification of Gallic acid, Quercetin and Glycyrrhizin equivalents in the methanolic extracts of Abrus precatorius (MEAP) and Cordia wallichi leaves (MECW). Material and Methods: The RP-HPLC method was carried out in reverse phase C18 column, (5 µm, 250mm x 4.6mm i.d) Dionex Ultimate 3000 liquid chromatography and detection was carried out at 254nm, using a mobile phase of Water: Acetonitrile: Methanol (pH 3.5) (15:15:70 v/v/v) with Isocratic reverse phase technique. Results: The experimental results showedthe amount of Gallic acid in the methanolic extracts of A. precatorius and C. wallichi leaves (0.63% and 0.36% respectively) , Quercetin(0.16% and 0.24% respectively), and Glycyrrhizin 0.11% in the extract of A. precatorius only. The high percentage of recovery (96-103%), low coefficient of variation (R²> 0.99) and low limit of detection (LOD), and limit of quantitation (LOQ) confirm the suitability of the method for simultaneous quantification of these three biomarkers in the two plants under investigation. Conclusion: This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the herbal product.