RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF LORNOXICAM AND EPERISONE HYDROCHLORIDEAbstract
Several spectrophotometric and HPLC methods for the determination of lornoxicam and eperisone hydrochloride have been documented individually or in combination with other drugs in pharmaceutical dosage forms. Therefore, in the present study, a reasonable and practical reverse phase HPLC method was developed and validated for lornoxicam and eperisone hydro-chloride in bulk formulation. In RP-HPLC method, the analyte was resolved by using an isocratic system, Methanol: Water (55:45) with 0.1% v/v Ammonium Hydroxide, pH 7.4 was used as mobile phase, at a flow rate of 1.0 ml/min, on HPLC system containing C18 analytical column (25 × 0.46 cm, 5 µm). The detection was carried out at 274 nm. The retention time was found to be 7.4 min and 9.2 min for lornoxicam and eperisone hydrochloride, respectively. Good resolution and retention time were observed. The results of the analysis in the method were validated with respect to following parameters linearity, precision, specificity and system suitability, limit of detection and limit of quantification.
Z. Bohra *, V. Jain and N. Singh
Department of Pharmaceutical Chemistry, NRI Institute of Pharmacy, Bhopal, Madhya Pradesh, India.
23 July 2020
16 December 2020
15 May 2021
01 July 2021