RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF STABILITY INDICATING METHOD A FOR ESTIMATION OF LOSARTAN POTASSIUM UNDER STRESS CONDITION AND TABLET DOSAGE FORM
AbstractLosartan potassium, a highly effective blood pressure lowering agent, has widely used for treatment of hypertension. Simple, economic, selective, and precise and stability indicating HPLC method has been developed and validated for analysis of losartan potassium in bulk drug and formulation dosage form. In order to optimize more one response at time, the chemo metric approach which includes 23 factorial design at two level with three factors was set up to standardize the chromatographic condition. The mobile phase was 40:60 ACN: Buffer consist of 0.05% of orthophosphoric acid and 0.05% Triethylamine at flow rate 1.0ml/minute the eluent was monitored at 225nm. The calibration plots constructed using the concentration of 6.4 to 9.6µg/ml (80 -120%) with r2 =0.999, recovery =99.44% was indicative of accuracy of proposed method. The precision was calculated as repeatability, intraday (RSD) for the drug
Article Information
9
2343-51
559
1643
English
IJPSR
Amna B.W.E Mohammed* and Elsadig H.Rudwan
College of Animal Production science and Technology, Sudan University of Science and Technology, Sudan
ehrrajab@hotmail.com
29 December, 2015
03 February, 2016
03 April, 2016
10.13040/IJPSR.0975-8232.7(6).2343-51
01 June 2016
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