RP-HPLC METHOD FOR SIMULTATANEOUS DETERMINATION OF IRBESARTAN, LOSARTAN, HYDRO-CHLOROTHIAZIDE AND CHLORTHALIDONE–APPLICATION TO COMMERCIALLY AVAILABLE DRUG PRODUCTSAbstract
A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-hypertensive drugs Irbesartan, Losartan, diuretics Hydrochlorothiazide and Chlorthalidone. The separation was achieved on Hypersil BDS (Length 250 mm × Diameter 4.6 mm Particle size 5 μm) column with gradient flow. The mobile phase at a flow rate of 1.0 mL min−1 consisted of 0.05 M sodium dihydrogen phosphate buffer and acetonitrile (Gradient ratio). The UV detection was carried out at 220 nm. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage form.
R. A. Mhaske*, S. Sahasrabudhe and A. A. Mhaske
Asst General Manger – AMD Sharon Bio-Medicine Ltd., Plot L6, Taloja-MIDC, Taluka – Panvel, Dist Raigad, 410208, Maharashtra, India
19 December, 2011
12 January, 2012
28 March, 2012
01 April, 2012