SCREENING OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF GLORIOSA SUPERBA IN RODENTS
HTML Full TextSCREENING OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF GLORIOSA SUPERBA IN RODENTS
B. Srinivasa, Basavaraj C. Kotinatot, Aruna Bushan and P. Madhav *
Department of Pharmacology, Apollo Institute of Medical Sciences and Research, Chittoor, Andhra Pradesh, India.
ABSTRACT: Background: Traditionally Gloriosa superba is being used as a medicinal plant in outskirts of belagavi, we evaluated and compared the analgesic and anti-inflammatory activities of hydroalcoholic extract of dried aerial parts (rhizomes) of G. superba with standard drugs. Methods: This experimental animal study was carried out in the Research Laboratory, Department of Pharmacology, BIMS, Belagavi. Prior permission from institutional ethical committee and institutional animal ethics committee was taken. Albino rats and Albino mice were used. Acute toxicity study was also done according to OECD guideline no. 425 and test doses were decided accordingly. The experimental models of Eddy’s hot plate method was used to study the analgesic activity whereas formalin induced peritonitis and cotton pellet granuloma models were used for anti-inflammatory action. Statistical analysis was performed using Mann-whitney U test and chi-square test (SPSS software version 22). Results: In eddy’s hot plate method, for G. superba extract of 400mg/kg, percentage increase in reaction time of at 120 mins was 60.49%, in formalin induces peritonitis method, for G. superba extract of 400mg/kg, percentage inhibition of peritoneal exudate was 47.14%. In cotton pellet induced granuloma method, for G. superba extract of 400mg/kg, percentage inhibition of granuloma formation was 50.32%. All the results of 4 different groups in each method showed significant difference in their respective pharmacological activities (Chi Square values: 14.4, 14.4, 75.2). Conclusion: Hydroalcoholic extract of G. superba has shown significant analgesic and anti-inflammatory activity in our study.
Keywords: Analgesic activity, Anti-inflammatory activity, Gloriosa superba
INTRODUCTION: Pain is an unpleasant sensation and emotional experience associated with actual or potential tissue damage or described in terms of such damage 1. The pathophysiology of pain involves 2 components, peripheral nociception and central mechanism.
There are 2 main classes of pain - Integumental pain and visceral pain 2. Treatment available to reduce the pain includes NSAIDS, opioids, anticonvulsants and muscle relaxants and these drugs also have adverse effects which are common nowadays.
Inflammation is a normal protective response to tissue injury caused by physical trauma, noxious chemicals or microbiologic agents 3. Drug therapy of inflammation has always been debatable. The drugs most commonly used now in the treatment of inflammation are glucocorticoids and non-steroidal Anti-inflammatory drugs (NSAIDS). Most of these drugs are known to cause more or less deleterious side effects even leading to hospital admissions due to ADRs. Hence there is an always scope for continuous research to identify more effective and safer agents in the therapy of inflammation which are potent and nontoxic. Indian traditional system of medicine uses herbs that have anti-inflammatory property. Gloriosa superb is one of many medicinal plants extensively used in southern parts of India for treatment of diverse conditions predominantly pertaining to pain and inflammation. Gloriosa superb is a good abortifacient 4. Its seeds and tubers are used mainly for treating gout and rheumatism 5. Roots are purgative, cholagogue, antihelminthic, astringent and germicidal. It also cures leprosy, swelling, piles, chronic ulcers and colic pain in bladder. Powder is used for treatment of rheumatic fever. Various parts of the plant are used in sores, tumours and syphillis. Extract of plant is also used as CNS depressant 6. Paste is an antidote in snake bite 7.
Scientific basis for traditional use of this plant is not much explored. Hence, the study is chosen to screen the analgesic and anti-inflammatory activity of hydro alcoholic extract of rhizome of Gloriosa superb through analgesic model, acute and subacute inflammatory models and to provide pharmacological basis for its use in traditional medicine for various analgesic and inflammatory disorders.
FIG. 1A:
MATERIALS AND METHODS: Our study was carried out in the Research Laboratory, Department of Pharmacology, Belagavi Institute of Medical Sciences, Belagavi. Ethical clearance was taken before commencing the study and CPCSEA number stated below.
Collection of Plant Product: Rhizomes of G. superba were collected from the local ayurvedic pharmacy of Belagavi, Karnataka, India and authenticated by an authorized person in botany. The dried parts of rhizomes were powdered and stored in an air tight container.
Preparation of Extract: The powder (2kg) was soaked in 50% ethanol and 50% distilled water (i.e. hydroalcoholic extract) at 50-55°C for 7 days. After 7days, solvent from the total extract was distilled off and the concentrate was evaporated on a water bath to a syrupy consistency and then evaporated to dryness 8.
Selection of Animals: Wistar albino rats of either sex weighing between 150 and 200 g and albino mice of either sex weighing between 20 and 30 g were obtained from BIMS animal house registered under the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) and maintained under good laboratory conditions. These animals were used for the acute toxicity, anti-inflammatory and analgesic activities. The study was approved by Institutional Ethics Committee for animal experimentation (Approval no: BIMS/IAEC/PG/06/2018 Dated- 13/11/2018).
The animals were stabilized for 1 week, maintained under standard laboratory conditions and were given standard pellet diet and water ad libitum throughout the course of the study. The animals were handled gently to avoid undue stress, which could result in an increased adrenal output.
Acute Toxicity Studies: Toxicity study for hydroalcoholic extract of G. superba was carried out as per OECD guideline number 425 9. Healthy adult albino wistar rats (200-250 g) were used. Animals were fasted overnight prior to dosing. The body weight of each animal was determined to calculate the dose. Hydroalcoholic extract of G. superba was administered in the dose of 2000 mg/kg body weight orally to one animal which survived. Thereafter, four other animals were dosed sequentially. All the animals were closely observed for 14 days. As no fatality was observed, LD50 was estimated to be greater than 2000 mg/kg. After performing a pilot study, we decided to use 200 mg/kg and 400mg/kg of hydroalcoholic extract G. superba for all the experiments.
Screening Methods for Analgesic Activity:
Eddy’s Hot Plate Method: Mice of either sex were weighed and divided into 4 different groups (n = 6 in each group). Group I served as control. Group II (Pentazocine 46.8 mg/kg body weight) served as standard and groups III and IV were treated with extracts at a dose of 200 and 400 mg/kg body weight, respectively. The reaction time of animals was noted down on hot plate at 0, 30, 60, 90, 120 and 150 minutes after the treatment. The basal reaction was the time taken by observing hind paw licking or jump response (whichever appeared first) in animals while placed on hot plate, which was maintained at constant temperature 55° C. A cut off period of 10 seconds was observed to avoid damage to the paws. The percentage increase or decrease in reaction time as index of analgesia at each time interval was calculated 10.
Percentage increase in reaction time = {(Rt / Rc) – 1} x 100
Where Rt is reaction time in treated group and Rc is reaction time in control group.
Screening Methods for Anti-Inflammatory Activity: Rats were randomly divided into four groups of 6 each. Group I served as control. Group II (diclofenac 13.5 mg/kg body weight) served as standard and groups III and IV were treated with G. superba extracts at a dose of 200 and 400 mg/kg body weight, respectively. Each rat was fed with respective drug one hour prior to the administration of phlogestic agent.
Formalin Induced Peritonitis in Rats: The method described by Teotino et al 11 was used to study the acute inflammatory reaction induced by intraperitoneal injection of formalin in rats. Under aseptic precautions, 1ml of 1% formalin was injected intraperitoneally in each rat. At the end of four hours, peritoneal exudates was collected by opening the ventral abdominal wall. Volume of the peritoneal exudates in diclofenac (13.5mg/kg) and G. superba treated group was compared with the control group.
Percentage inhibition of peritoneal exudate formation in diclofenac and G. superba treated group was determined by the following formula.
Percentage inhibition of peritoneal exudates = {1-(V t/ Vc)} х100
Where, Vc- Mean volume of exudates in control group.
Vt- Mean volume of exudates in treated test groups.
Cotton Pellet Induced Granuloma in Rats: The method described by Meier et al 12 was used to study the chronic inflammatory reaction induced by subcutaneous cotton pellet implantation. Sterile cotton pellets weighing 20 mg each was implanted subcutaneously in the axillary region of both forefeet and groin under anaesthesia 13.
Each rat was orally administered with respective drug once daily for 7 consecutive days.
On 8th day, the animals were sacrificed, cotton pellet with granulation tissue was removed, dried at 60°C in hot air oven for 24 hours and dry weight was determined.
Percentage inhibition of granuloma formation in diclofenac (13.5mg/kg) and Gloriosa superba treated rats was determined by the following formula:
Percentage inhibition of granuloma formation = {1-(Tt / Tc)} х 100
Where, Tc- Weight of granuloma tissue in control group
Tt- Weight of granuloma tissue in treated test groups
RESULTS: Results were analyzed using Mann-whitney U test and chi-square test (SPSS software version 22). Table 1 and Figure 1 indicate that hydroalcoholic extract of rhizomes of Gloriosa superba in dose of 200mg/kg and 400mg/kg shows significant analgesic activity with percentage increase in reaction time of 47.84% & 60.84% respectively in eddy’s hot plate method. Further, above two test doses of 200mg/kg and 400mg/kg of Gloriosa superba when compared with standard drug – Pentazocine (79.78%), showed no significant difference in analgesic activity (P=0.18 - P ˃ 0.05, P = 0.748 - P ˃ 0.05). Thus, test drug activity is not comparable with the standard drug pentazocine (P ˃ 0.05). Analgesic activity of two test doses of test drug (200mg/kg and 400mg/kg) was not comparable (P = 0.407 - P ˃ 0.05).
All the results of four groups showed significant difference in analgesic activity (Chi square = 14.412).
Table 2 and Figure 2 indicate that hydroalcoholic extract of rhizomes of Gloriosa superba in dose of 200mg/kg and 400mg/kg shows significant anti-inflammatory activity with percentage inhibition in peritoneal exudates of 40.35% & 47.14% respectively in acute model of inflammation. Further, above two test doses of 200mg/kg and 400mg/kg of Gloriosa superba when compared with standard drug Diclofenac (49.28%), showed no significant difference in anti-inflammatory activity (P=0.145 - P ˃ 0.05, P = 0.63 - P ˃ 0.05). Thus, test drug activity is not comparable with the standard drug diclofenac (P ˃ 0.05).
Anti-inflammatory activity of two test doses of test drug (200mg/kg and 400mg/kg) was not comparable (P = 0.256 - P ˃ 0.05). All the results of four groups showed significant difference in anti-inflammatory activity (Chi square = 14.441). Table 3 and Fig. 3 indicate that hydroalcoholic extract of rhizomes of Gloriosa superba in dose of 200mg/kg and 400mg/kg shows significant anti-inflammatory activity with percentage inhibition in granuloma formation of 35.54% & 50.32% respectively in sub-acute model of inflammation. Further, above two test doses of 200mg/kg and 400mg/kg of G. superba when compared with standard drug – Diclofenac (55.98%), showed significant difference in anti-inflammatory activity (P=0.001 - P ˂ 0.05, P = 0.004 - P ˂ 0.05). Thus, test drug activity is comparable with the standard drug diclofenac (P ˂ 0.05). Anti-inflammatory activity of two test doses of test drug (200mg/kg and 400mg/kg) was comparable or statistically significant (P = 0.001 - P ˂ 0.05). All the results of four groups showed significant difference in anti-inflammatory activity (Chi square = 75.174).
TABLE 1: ANALGESIC EFFECT OF VARIOUS DRUGS ON MICE USING EDDY'S HOT PLATE METHOD
Groups | Dose | Reaction time in seconds | Mean reaction time in seconds +/-SEM | Percentage (%) increase in reaction time | |||
0 min | 30 min | 60min | 120 min | ||||
Control | - | 3.0 ± 0.29 | 3.3 ± 0.19 | 3.17 ±0.38 | 3.5 ± 0.22 | 3.2 ± 0.27 | - |
Standard
(Pentazocine) |
46.8 mg/kg | 2.8 ± 0.25 | 4.5 ± 0.37 | 6.8 ± 0.52 | 9.1 ± 0.25 | 5.8 ± 0.35 | 79.78 |
Test 1 | 200 mg/kg | 3.0 ± 0.29 | 4.0 ± 0.29 | 5.8 ± 0.25 | 6.3 ± 0.19 | 4.7 ± 0.26 | 47.84 |
Test2 | 400 mg/kg | 3.2 ± 0.24 | 4.6 ± 0.29 | 5.9 ± 0.12 | 7.1 ± 0.39 | 5.2 ± 0.17 | 60.49 |
TABLE 2: ANTI-INFLAMMATORY EFFECT OF VARIOUS DRUGS ON RATS USING FORMALIN INDUCED PERITONITIS METHOD
Groups | Dose | Volume of peritoneal exudates (ml) | Mean peritoneal exudates volume (ml) ± SEM | % Inhibition |
Control | - | 2.6, 2.8, 3.2, 3.0, 2.7, 2.5 | 2.80 ± 0.09 | 0 |
Standard (Diclofenac) | 13.5 mg/kg | 1.6, 2.0, 1.4, 1.3, 1.2, 1.0 | 1.42 ± 0.11 | 49.28 |
Test 1 | 200mg/kg | 1.4, 1.4, 1.8, 1.8, 2.0, 1.6 | 1.67 ± 0.09 | 40.35 |
Test 2 | 400mg/kg | 1.7, 1.8, 1.5, 1.5, 1.2, 1.2 | 1.48 ± 0.08 | 47.14 |
TABLE 3: ANTI-INFLAMMATORY EFFECT OF VARIOUS DRUGS ON RATS USING COTTON PELLET INDUCED GRANULOMA METHOD
Groups | Dose | Dry granuloma weight in
(mg) |
Mean dry granuloma Weight (mg) ± SEM | % Inhibition occurred |
Control | - | 17, 19, 19, 19, 18, 16, 18, 18, 18, 18, 16, 16, 20, 18, 19, 21, 20, 20, 18, 18, 20, 20, 19, 18 | 18.46 ± 0.36 | 0 |
Standard
(Diclofenac) |
13.5 mg/kg | 5, 7, 6, 6, 6, 8, 7, 6, 7, 9, 8, 9, 7, 6, 9, 9, 8, 8, 9, 6, 11, 9, 10, 9 | 8.125 ± 0.37 | 55.98 |
Test 1 | 200mg/kg | 12, 11, 11, 12, 13,11, 10, 12, 14, 10, 12, 12, 12, 10, 12, 12, 10, 10, 12, 24, 16, 10, 12, 16 | 11.9 ± 0.25 | 35.54 |
Test 2 | 400mg/kg | 10, 8, 7, 9, 11, 6, 7, 8, 9, 8, 7, 7, 12, 10, 9, 10, 10, 11, 9, 10, 12, 11, 9, 10 | 9.17 ± 0.48 | 50.32 |
TABLE 4: REPORTED ADDITIONAL SPECIAL PHARMACOLOGICAL PROPERTIES OF DIFFERENT PARTS OF GLORIOSA SUPERBA WITH DIFFERENT EXTRACTS
Extract | Parts used | Activity | References |
Chloroform and N-butanol | Leaves, tubers, seeds | Anti-microbial and Anti-cancer | 27 |
Methanolic, aqueous and petroleum ether | Tubers | Anti-bacterial, Anti-fungal, mutagenic | 28 |
Acetone | Tubers and stem | Anti-fungal | 29 |
Alcoholic | Tubers | Anti-microbial | 30 |
Alcoholic | Tubers | Anti-helminthic | 30 |
Ethanol and water | Whole plant | Anti-helminthic | 31 |
Methanolic | Leaf and stem | Anti-oxidant and Anti-microbial | 32 |
Methanolic | Seeds, tubers and leaves | Anti-oxidant | 17 |
Methanolic, acetone and water | Tubers | Anti-oxidant | 33 |
Methanol, hexone, chloroform | Tubers and seeds | Anti-bacterial | 34 |
Acetone | Tubers and leaves | Anti-bacterial | 35 |
Alcoholic | Tubers | Anti-haemolytic | 36 |
Aqueous | Leaves | Anti-thrombolytic and Anti-coagulant | 37 |
FIG. 1: SHOWING PERCENTAGE (%) INCREASE IN REACTION TIME IN EDDY’S HOT PLATE MODEL
FIG. 2: SHOWING PERCENTAGE (%) INHIBITION OF MEAN VOLUME OF PERITONEAL EXUDATES IN FORMALIN INDUCED PERITONITIS MODEL
FIG. 3: SHOWING PERCENTAGE (%) INHIBITION OF GRANULOMA FORMATION IN COTTON PELLET INDUCED GRANULOMA MODEL
DISCUSSION: In the present era there are number of different inflammatory disorders for which there are variety of anti-inflammatory drugs but associated with side effects like gastric ulcers, GI bleeding, renal damage etc. So, anti-inflammatory drugs with minimal side effects to be explored therapeutically in huge magnitudes for variety of inflammatory disorders and used accordingly.
In this context, it is exciting to note the reports of medicinal values of several indigenous plant preparations. Uses of these preparations are also consistently highlighted in ayurveda but without proper scientific knowledge. Hence, some of different extracts of different plants have been tested for pharmacological activities.
Globally there is extensive traditional use of a plant by name “Gloriosa superba” as there is significant therapeutically potential like analgesic, anti-rheumatic, anti-pyretic activities etc. Thereby we tried to evaluated the analgesic and anti-inflammatory activities of this plant by specific models respectively as described above to support and provide pharmacological basis in future for the use in our allopathy practice.
In Eddy’s hot plate model the extract of G. superba increased reaction time from 40.84% in test dose 200mg/kg to 60.49% in 400mg/kg to thermal pain, which simulates central antinociceptive test 14 and thereby standard drug pentazocine was used to compare and simulate central antinociception with test drug. Inhibition of histamine or kinin pathway may reduce pain. The results of the present study also showed that extract exhibited a comparable magnitude of antinociceptive activity in both models of pain which suggested that the phytochemical constituents are responsible for the analgesic effect. The analgesic activity of some flavonoids 15 and terpenoids already has been reported suggesting that these or similar constituents may be responsible for the analgesic effect of the extract. The results of the present study indicated that the hydroalcoholic extract of G. superba might contain constituents capable of relieving or modifying responses to pain caused by either thermal or chemical stimulation of the noiceptors mediated by both central and peripheral mechanisms. Both the two doses of extract exhibited maximum percentage protection due to increase in reaction time (6.30 ± 0.19, 7.16 ± 0.39) at 90 minutes after drug administration thereby clearly indicating good analgesic activity of Gloriosa superba. The results obtained from this study is similar to study done by John et al 16 and support our study.
Formalin induced peritonitis model effectively demonstrates the use of formalin to induce peritonitis in rats by injecting intraperitoneally and results showed significant effect of standard anti-inflammatory drug diclofenac and Gloriosa superba as there was significant percentage protection or percentage inhibition in the volume of peritoneal exudates (40.34%. with G S 200mg/kg and 47.14%. with G S 400mg/kg) when compared to control thereby clearly indicating anti-inflammatory activity of Gloriosa superba.
Formalin was choosen as the preferred phlogestic agent to induce acute inflammation because it adequately resembles the chain of events underlying inflammation in patients. Other phlogestic agents which can be used to induce acute inflammation are carrageenan (sulphated polysaccharide obtained from seaweed Rhodophyceae), lipopolysaccharide, zymosan based on varying degree and duration of action needed. As there are no similar studies done of this model for this plant G. superba, comparison cannot be done.
Cotton pellet induced granuloma model effectively demonstrates and measures both exudative and proliferative phases of inflammation. 200mg/kg of G. superba extract showed statistically significant decrease in dry granuloma weight (35.54%, P=0.001 hence P ˂ 0.05) when compared to control group. This shows that hydroalocoholic extract of this plant is effective against subacute and chronic inflammation but its activity was inferior in the dose of 200mg/kg when compared to standard drug diclofenac. 400mg/kg dose of this plant extract showed statistically significant decrease in dry granuloma weight (50.32%, P=0.001 hence P ˂ 0.05) when compared to control group. The activity of G. Superba in 400mg/kg was almost similar to standard drug diclofenac. The results obtained from this model for G. superba is slightly superior to study done by John, et al 16 and support the study. As there are very few studies done previously of this model for this plant, our results cannot be compared with other studies. Because medicinal plants are rich in flavonoids and every group of flavonoids has a capacity to act as antioxidants 17, different studies of various parts of this plant has been demonstrated the scientific basis for analgesic and anti-inflammatory activity by isolation of various chemical constituents of this plant. Among them, flavones and catechin components acting as most powerful flavonoids for protecting the body against ROS 18.
The other flavonoid components such as quercetin, kaempferol, myricetin and rutin have antioxidant, anti-inflammatory, antiviral and antiallergic, as well as anticancer activities 19, 20, G. superba tubers contain colchicines, benzoic and salicylic acid, sterols and resinous substances like as colchicines, 3-demethyl colchicine, 1,2-didemethyl colchicine, 2, 3-didemethyl colchicine, N-formyl, N-deacetyl colchicines, colchicocide, gloriosine, tannins and superbine 21.
Colchicine is the major compound isolated from the seed and rhizome of this plant 22 and other important compound is gloriosine 23, 24, In addition, G. superba tubers hold 0.25% colchicine apart from containing sitosterol, glucoside, β-and gamma lumicolichicines, β-sitosterol, flucoside and 2-H-6-MeO benzoic acid and flowers contain luteolin and N-formylde-Me-Colchicine 25, 26. Apart from these important phytochemical constituents of G. superba and their respective pharmacological properties, there are more additional special properties of this plant with different extracts which are extensively explored and documented by various reports of different studies (As mentioned in Table 4. In our study we conclude scientifically that rhizomes of Gloriosa superba showed good analgesic & anti-inflammatory activity in mice & rats.
ACKNOWLEDGEMENT: We thank statistician and director of our institution for their contribution in statistics of our study & granting permission to conduct the study in the institution respectively.
CONFLICTS OF INTEREST: NIL
REFERENCES:
- Benett PN and Brown MJ: Clinical pharmacology. 12th edition. Spain; Elsevier 2018; 357.
- Sharma HL and Sharma KK: Principles of Pharmacology. 4th edition. Hyderabad; Paras Medical Publishers 2023; 371.
- Whalen K: Lippincott’s Illustrated Reviews: Pharmacology. South Asian edition. New Delhi; Wolters Kluwer 2019; 693.
- Malpani AA, Aswar UM, Kushwaha SK, Zambare GN and Bodhankar SL: Effect of the Aqueous Extract of Gloriosa superba Linn (Langli) Roots on Reproductive System and Cardiovascular Parameters in Female Rats. Trop J Pharm Res 2011; 10(2): 169-176.
- Chatterjee T and Ghosh B: Gloriosa: A High Demanding Pharmaceutical Plant in Near Future. International Journal of Economic Plants 2015; 2(2): 089-102.
- Shifila AN and Varkey J: Evaluation of Anxiolytic Activity of Ethanolic Extract of Tubers of Gloriosa superba Linn in Mice. IJPSRR 2022; 75(1): 184-190.
- Meshram SM, Mohture VM and Kayarkar AR: Traditional Herbal Medicines for the Treatment against Snake Bite. International Journal for Innovative Research in Multidisciplinary Field 2021; 7(10): 93-97.
- John JC, Fernandes J, Nandgude T, Niphade SR and Deshmukh PT: Analgesic and Anti-inflammatory activities of the Hydroalcoholic extract from Gloriosa superba IJGP 2009; 3(3): 215-9.
- OECD/ OCD. 425 OECD Guidelines for testing of chemicals acute oral toxicity up and down procedure 2001; 26: 1-26.
- Argal A and Pathak AK: CNS activity of Calotropis gigantea J Ethnopharmacol 2006; 106: 142–5.
- Teotino UM, Polofrig L and Gandini A: Antipyretic activity of thio derivatives of 2,3-dihydro 4H-1,3-Benzoxacin-4-1 synthesis and pharmacological property. J Med Chem 1963; 6: 248-9.
- Meier R, Schuler W and Desaulles P: On the mechanism of cortisone inhibition of connective tissue proliferation. Experientia 1950; 6(12): 469-71.
- Goldstein SA, Shemano I, Daweo R and Betler JM: Cotton pellet granuloma pouch method for evaluation anti inflammatory activity. Arc Intl Pharmcodyn Ther 1967; 165: 294-301.
- Della LA, Tubaro A, Dri P, Zilli C and Del NP: The role of flavonoids in the anti-inflammatory activity of Chamomaiia recutita. Clin Biol Res 1968; 213: 481-6.
- Dirosa M: Biological properties of carrageenin. J Pharm Pharmacol 1972; 24: 89-102.
- Jomy C John, Jennifer Fernandes, Tanaji Nandgude, Samir R Niphade, Alok Savla and Pradeep T: Deshmukh: Analgesic and anti-inflammatory activities of Gloriosa superba International Journal of Green Pharmacy 2009; 1: 215-219. DOI: 10.4103/0973-8258.56277
- Saradha Devi M, Ashokkumar K and Annapoorani S: Identification and determination of flavonoids, carotenoids and chlorophyll concentration in Cynodon dactylon (L.) by HPLC analysis. Natural Products Research 2015; 29(8): 785-790.
- De Groot H: Reactive oxygen species in tissue injury. Hepatogastroenterology 1994; 41: 328–332.
- Fraga CG, Mactino US, Ferraro GE, Coussio JF and Boveris A: Flavonoids as antioxidants evaluated by in-vitro and in-situ liver chemiluminescence. Biochem Pharmacol 1987; 36: 717–720.
- Halliwell B: Free radicals, antioxidants and human disease: curiosity, cause or consequence?. Lancet 1994; 344: 721–724.
- Capraro HG and Brossi A: The alkaloids. 1st edition. New York; Arnold Brossi Academic Press 1984; 23: 1-70.
- Sarin YK, Jamwal PS, Gupta BK and Atal CK: Colchicine from the seeds of Gloriosa superba. Curr Sci 1974; 43: 87-90.
- Gooneratne BW: Massive generalized alopecia after poisoning by superba Linn. British Medical Journal 1966; 231(5494): 1023-4.
- Angunawela RM and Fernando HA: Acute ascending Polyneurotpathy and dermatitis following poisoning by tubers of superba Linn. Ceylon MJ 1971; 16(4): 233-5.
- Veeraiah S and Jaganmohan Reddy K: Current strategic approaches in ethanomedicinal plants of Tinospora cordifolia and Gloriosa superba - a review. International Journal of Pharma and Bio Sciences 2012; 3(2): 320-326.
- Suri OP, Gupta BD and Suri KA: A new glycoside, 3-Odemethylcolchicine-3-O-alpha-d-glucopyranoside from Gloriosa seeds. Natural Product Letters 2001; 15: 217-219.
- Budhiraja A, Nepali K, Kaul S and Dhar KL: Antimicrobial and cytotoxic activities of fungal isolates of medicinal plant Gloriosa superba. Int J Recent Adv Pharm Res 2012; 2(1): 37-45.
- Hemaiswarya S, Raja R, Anbazhagan C, Thiagarajan V. Antimicrobial and mutagenic properties of the root tubers of Gloriosa superba Linn (Kalihari). Pak J Bot 2009; 41(1): 293-299.
- Kamna B and Anirudha R: Antimicrobial efficacy of an endemic plant species (Gloriosa superba). Int J Pharm Bio Sci 2012; 3(4): 353-359.
- Suryavanshi S, Rai G and Malviya SN: Evaluation of anti-microbial and antihelmintic activity of Gloriosa superba Advance Research in Pharmaceuticals and Biologicals 2012; 2(1): 45-52.
- Bhushan P, Vishal W, Nayana P, Mohan A and Prashant S: Anthelmintic Activity of Gloriosa superba Linn (Liliaceae). Int. J. Pharm. Tech. Research 2010; 2(2): 1483-1487.
- Moteriya P, Ram J, Rathod T and Chanda S: In-vitro Antioxidant and Antibacterial Potential of leaf and stem of Gloriosa superba American Journal of Phytomedicine and Clinical Therapeutics 2014; 2(6): 773-787.
- Jagtap S and Satpute R: Phytochemical screening, antioxidant, antimicrobial and flavonoid analysis of Gloriosa superba Rhizome extracts. Journal of Academia and Industrial Research 2014; 3(6): 247-254.
- Senthilkumar M: Phytochemical screening and antibacterial activity of Gloriosa superba International Journal of Pharmacognosy and Phytochemical Research 2013; 5(1): 31-36.
- Haroon RB and Nagarajan N: Antibacterial Potential of Glory lily Gloriosa superba International Research Journal of Pharmacy 2011; 2(3): 139-142.
- Kumarapppan C, Jaswanth A and Kumarasunderi K: Antihaemolytic and snake venom neutralizing effect of some Indian medicinal plants. Asian Pacific Journal of Tropical Medicine 2011; 4(9): 743-747.
- Kee NLA, Mnonopi N, Davids H, Naude RJ and Frost CL: Antithrombotic/anticoagulant and anticancer activities of selected medicinal plants from South Africa. African Journal of Biotechnology 2008; 7: 217-223.
How to cite this article:
Srinivasa B, Kotinatot BC, Bushan A and Madhav P: “Screening of analgesic and anti-inflammatory activity of Gloriosa superba in rodents”. Int J Pharm Sci & Res 2023; 14(12): 6000-07. doi: 10.13040/IJPSR.0975-8232.14(12).6000-07.
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IJPSR
B. Srinivasa, Basavaraj C. Kotinatot, Aruna Bushan and P. Madhav *
Department of Pharmacology, Apollo Institute of Medical Sciences and Research, Chittoor, Andhra Pradesh, India.
madhavmbbs47@gmail.com
05 May 2023
26 June 2023
05 July 2023
10.13040/IJPSR.0975-8232.14(12).6000-07
01 December 2023