SIMPLE AND SENSITIVE VALIDATED REVERSE PHASE HPLC-UV METHOD FOR THE DETERMINATION OF LYMECYCLINE IN PHARMACEUTICAL DOSAGE FORMS
AbstractA Simple, Sensitive, Precise, and specific reverse phase high performance liquid chromatographic method has been developed for the determination of lymecycline in pharmaceutical Dosage Forms. Chromatographic separation was achieved on a PLRP-S (250×4.6 mm), 8.0 µm make: Varian column with a 11.5:10:20:1:57.5 mixture of 2-Methyl-2-propanol,3.5% w/v di-potassium hydrogen phosphate, 1.0% w/v Tetra butyl ammonium hydrogen sulphate, 4.0% w/v di -sodium edetate and JT Baker water as mobile phase, detection was at 254 nm. Response was a linear function of concentration in the range 5-0.02 mg/L for lymecycline; correlation coefficient was 0.9998, respectively. LOD and LOQ for lymecycline were found 0.02 mg/L and 0.05 mg/L. Accuracy (recoveries 95-97%) and reproducibility were found to satisfactory.
Article Information
63
2301-2305
467KB
2009
English
IJPSR
T. Nageswara Rao, T.B. Patrudu , T. Srinivasa Rao and T. Parvathamma
Research Scholar, Department of Analytical Chemistry, International Institute of Biotechnology and Toxicology (IIBAT), Padappai-601 301, Kanchipuram District, Tamil Nadu, India
26 March, 2012
25 June, 2012
28 June, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(7).2301-05
01 July 2012