SIMPLE AND SENSITIVE VALIDATED REVERSE PHASE HPLC-UV METHOD FOR THE DETERMINATION OF LYMECYCLINE IN PHARMACEUTICAL DOSAGE FORMSAbstract
A Simple, Sensitive, Precise, and specific reverse phase high performance liquid chromatographic method has been developed for the determination of lymecycline in pharmaceutical Dosage Forms. Chromatographic separation was achieved on a PLRP-S (250×4.6 mm), 8.0 µm make: Varian column with a 11.5:10:20:1:57.5 mixture of 2-Methyl-2-propanol,3.5% w/v di-potassium hydrogen phosphate, 1.0% w/v Tetra butyl ammonium hydrogen sulphate, 4.0% w/v di -sodium edetate and JT Baker water as mobile phase, detection was at 254 nm. Response was a linear function of concentration in the range 5-0.02 mg/L for lymecycline; correlation coefficient was 0.9998, respectively. LOD and LOQ for lymecycline were found 0.02 mg/L and 0.05 mg/L. Accuracy (recoveries 95-97%) and reproducibility were found to satisfactory.
T. Nageswara Rao, T.B. Patrudu , T. Srinivasa Rao and T. Parvathamma
Research Scholar, Department of Analytical Chemistry, International Institute of Biotechnology and Toxicology (IIBAT), Padappai-601 301, Kanchipuram District, Tamil Nadu, India
26 March, 2012
25 June, 2012
28 June, 2012
01 July 2012