SIMPLE ISOCRATIC RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF GATIFLOXACIN IN TABLET DOSAGE FORMAbstract
The objective of the present study is to develop and validate a method for the estimation of gatifloxacin (GTX) in the tablet dosage form. An isocratic reversed-phase high-performance liquid chromatographic (HPLC) method was developed to estimate gatifloxacin using X terra C18 column (4.6 mm ´ 150 mm I.D., 5µm particle size) and the mobile phase containing phosphate buffer (pH adjusted to 3.5 with dilute phosphoric acid) and acetonitrile (60:40 v/v) at the flow rate of 0.5 ml/min. The UV detection was carried out at 298 nm. The retention time of gatifloxacin was found to be 2.340 min. The developed method was validated concerning accuracy, precession, linearity, selectivity, ruggedness, and robustness. Linearity for gatifloxacin was observed in the concentration range 0.01 to 0.50 µg/ml. Percent recovery was found to be 99.67. The results confirmed that the proposed method is specific, rapid, reproducible, and suitable for the routine determination of gatifloxacin.
A. K. Bera, A. K. De and B. Pal *
Department of Chemistry, St. Paul’s C. M. College, 33/1 Raja Rammohan Roy Sarani, Kolkata, West Bengal, India.
03 March 2014
02 May 2014
18 June 2014
01 September 2014