SIMULTANEOUS DETERMINATION OF ETODOLAC AND PARACETAMOL IN BULK DRUGS AND PHARMACEUTICAL FORMULATION BY HPTLC-DENSITOMETRIC AND UV-DERIVATIVE SPECTROPHOTOMETRY METHODS
AbstractIn current research work two methods have been developed for simultaneous determination of etodolac and paracetamol in binary mixtures. HPTLC-densitometry was employed for the determination of the mixture for etodolac 50-400 ng band⁻¹ and for paracetamol 50-300 ng band⁻¹. Separation was carried out on a silica gel 60 F254 HPTLC plates, using toluene: acetone: methanol: glacial acetic acid (6:2:1:0.5 v/v) as mobile phase. Etodolac, paracetamol and the toxic impurity para-aminophenol were well resolved with Rf values of 0.61±0.04, 0.41±0.04 and 0.20±0.03. Determination has been carried out at 254 nm with a mean percentage recovery of 99.77±1.30 for etodolac and 99.86 ± 0.97 for paracetamol. First derivative (D1) spectrophotometry was employed for simultaneous determination of etodolac (217nm) and paracetamol (236 nm). Linearity ranges of both the compounds were found to be 2.5-12.5µg mL⁻¹ with a mean percentage recovery of 98.07±1.62 for etodolac and100.65±0.84 for paracetamol respectively. Methods were validated according to ICH guidelines and successfully implemented for the analysis of bulk powder and pharmaceutical formulations.
Article Information
18
2056-2063
810
1725
English
IJPSR
Kalakotla Shanker *, Sanjay Kumar Kuna and Shashidhar Purra
Centre for Pharmaceutical Sciences, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad, Telangana, India.
shankerkalakotla@gmail.com
03 October, 2016
09 December, 2016
16 December, 2016
10.13040/IJPSR.0975-8232.8(5).2056-63
01 May, 2017
Download