SIMULTANEOUS DETERMINATION OF RESIDUAL NSAIDs AND ANTI-BIOTICS IN RAW MILK BY RP-HPLC
AbstractThe presence of Non-steroidal Anti-Inflammatory drugs (NSAIDs) and β-lactam residues in foodstuff constitutes a potential risk to the human health and undesirable effects on consumers. Now-a-days these drug residues are also recognized as an emerging environmental problem. The present work describes the development of a sensitive, reproducible, and accurate method using liquid chromatography with ultraviolet detector for simultaneous determination of the (NSAIDs) and β-lactam antibiotics (paracetamol, nimesulide, amoxicillin, and ampicillin) in buffalo raw milk. The extraction method involves liquid-liquid extraction using diethyl ether and centrifugation followed by evaporation under pressure using Nitrogen. The percentage recovery of the (NSAIDs) and β-lactam antibiotics was 85 and 90 % respectively with relative standard deviations between 0.4 and 0.9%. The limits of quantification (LOQs) for all these compounds were in the range of 1 to 3 ng/ml, which are lower than the Maximum Residue Limits (MRLs) established by the Food and Drug Administration (FDA) for the studied (NSAIDs) and β-lactams in milk, making the method suitable for performing routine analysis. The methods were validated according to Food and Drug Administration guide lines.
Article Information
57
5498-5502
579KB
1393
English
IJPSR
M. Pushpa Harshita*, J. Teja Aparna, L. Kumar Reddy, P. Venkata Suresh, A. Elphine Prabahar and N. Rama Rao
Department of Pharmaceutical Analysis, Chalapathi institute of pharmaceutical sciences, Lam, Guntur, Andhra Pradesh, India.
moparthyharshita@gmail.com
16 May, 2014
19 July, 2014
20 August, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(12).5498-02
01 December 2014
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