SIMULTANEOUS ESTIMATION OF DAPSONE AND ADAPALENE IN GEL FORMULATION BY UV- SPECTROSCOPYAbstract
Objective: A new, simple, sensitive, and economical UV spectro-photometric method was developed for the simultaneous analysis of Adapalene and Dapsone in pharmaceutical formulation. Method: This UV method was developed with Tetrahydrofuran and Distilled water as solvents. The wavelengths selected for analysis in the present method were 237 nm and 293 nm. The method was validated as per ICH guidelines. Results: The method was validated for linearity, accuracy, precision, specificity and robustness. Linearity was found to be within the concentration range of 0.05-0.25 µg/ml for Adapalene and 2.5-12.5 µg/ml for Dapsone. Accuracy for the method was determined by recovery studies. The % drug recovered was found to be 99-102% w/w. The % RSD values of repeatability and intermediate precision were found to be less than 2, providing method was precise in nature. From all these studies it was observed that there was no interference of excipients from the formulation during the analysis. Conclusion: The advantages of this method for analytical purposes lie in the rapid determination, its cost-effectiveness, easy preparation of the sample and good reproducibility. In addition to this, the present method can be recommended for the simultaneous determination of Adapalene and Dapsone in routine quality control analysis in combined drug formulations.
V. S. Vichare * and P. V. Handargule
PES Modern College of Pharmacy (for Ladies) Moshi, Pune, Maharashtra, India.
13 November 2019
20 July 2020
15 October 2020
01 December 2020