SIMULTANEOUS ESTIMATION OF SULFAQUINOXALINE SODIUM AND AMPROLIUM HYDROCHLORIDE BY RP- HPLC.Abstract
A new RP-HPLC method was developed and validated for the simultaneous determination of amprolium hydrochloride and sulfaquinoxaline sodium in a powder dosage form. The chromatographic separation was achieved by isocratic mode with Phosphate buffer and Acetonitrile (30:70; v/v), as mobile phase and using C-18 RP-Amide (25.0cm × 4.6 mmi.d, 5µ particle size) as stationary phase, with a flow rate of 1.0 ml/min at ambient temperature. The quantification of the analytes was monitored by UV detection at a wavelength of 263 nm. The optimized method was subjected to validation for specificity, accuracy, precision, linearity, range, ruggedness and robustness. The calibration graphs were linear in the concentration range of 0.08 – 0.12 mg/ml. The results were evaluated as per the International Conference on Harmonization (ICH) guidelines. The precision of the method in terms of RSD is not more than 2% for both the analytes. The method is sensitive, specific, fast and accurate with minimum sample preparation.
Abhilekha P Mantri*, Rubeena Mubeen S, Nischal Kandepu and Shiva Kumar
Provimi Animal Nutrition India Pvt Ltd, C7/22, KSSIDC Industrial Area, Yelahanka Newtown, Bangalore- 560 064, Karnataka, India
20 June, 2014
24 September, 2014
16 November, 2014
01 March, 2015