SIMULTANEOUS ESTIMATION OF TELMISARTAN, HYDROCHLORTHIAZIDE AND AMLODIPINE IN BULK AND SOLID DOSAGE FORM BY CHEMOMETRIC ASSISTED SPECTROPHOTOMETRIC METHODS

Chemometric designs were applied to develop a simple UV-visible spectroscopic method for the simultaneous estimation of Hydrochlorthiazide (HCT), Amlodipine (AMLO) and Telmisartan (TEL) in bulk and solid dosage form. The simultaneous spectroscopic method was developed for the three drugs and the data generated from the spectra were determined by using Chemometric methods such as trilinear regression analysis, Cramer’s matrix method, Method of least squares, Multivariate calibration methods such as partial least square regression (PLS) and Principle component regression (PCR). The wavelengths selected for all the above methods were 270 nm (wavelength of maximum absorption; λ_max of HCT), 342 nm (wavelength of maximum absorption; λ_max of AMLO) and 292 nm (wavelength of maximum absorption; λ_max of TEL). Results: The methods shows good linearity for TEL from 4 – 20 μg/ml, for HCT from 2-10 μg/ml and AMLO from 2 – 10 μg/ml with regression coefficient values of 0.970, 0.996 and 0.980 respectively. The RSD value for intraday and inter-day precision was found to be less than 2%. The percentage recovery and percentage assay was in the range of 95 – 105% for Telmisartan (TEL), Hydrochlorthiazide (HCT) and Amlodipine (AMLO) by all the methods. Conclusion: The developed methods neither require any oppressive separation procedure nor complex derivatization procedures for the analysis of the three drugs and moreover they are effective in minimizing the errors in analysis, simple and economical.