STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF FEXOFENADINE AND MONTELUKAST BY USING RP-UPLCAbstract
A simple, reliable, accurate and precise method was developed for the simultaneous estimation of Fexofenadine and Montelukast in pharmaceutical dosage form. The chromatogram was run through Standard HSS C18 (2.1 × 100 mm, 1.8 µm). Mobile phase employed was 0.1% OPA Buffer and Acetonitrile taken in the ratio of 65:35 was pumped through column at a flow rate of 0.2 ml/min. The temperature was maintained at 30 °C. The optimized wavelength selected was 269 nm. The retention time of Montelukast and Fexofenadine was found to be 0.921 and 1.101 min. The Percent RSD of the Montelukast and Fexofenadine were and found to be 0.7% and 0.4% respectively. The Percent recovery was obtained as 99.22% and 99.67% for Montelukast and Fexofenadine respectively. The LOD and LOQ values obtained from regression equations of Montelukast and Fexofenadine were 0.06, 0.18 and 0.80, 2.44 respectively. Regression equation of Montelukast was y = 6333.8x + 755.96 and y = 4038.3x + 8964.8 of Fexofenadine. The decrease in the retention and run times in this method that has been developed was simple and economical that can be been easily adopted in regular Quality control test in industries
Christina Emette Jaldi and Mangamma Kuna *
School of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, East Godavari District, Andhra Pradesh, India.
07 September 2020
12 February 2021
23 May 2021
01 September 2021