SYNTHESIS AND BIOLOGICAL EVALUATION OF NOVEL 6-(SUBSTITUTED BENZYLIDENE)-2-METHYLTHIAZOLO [2,3-b] OXAZOL-5(6H)-ONE AS POTENTIAL ANTICANCER AGENTS
HTML Full TextSYNTHESIS AND BIOLOGICAL EVALUATION OF NOVEL 6-(SUBSTITUTED BENZYLIDENE)-2-METHYLTHIAZOLO [2,3-b] OXAZOL-5(6H)-ONE AS POTENTIAL ANTICANCER AGENTS
Deepthi D. Kini * 1 and Jessy Elizabeth Mathews 2
Department of Pharmaceutical Chemistry 1, Karnataka College of Pharmacy, Bangalore - 560064, Karnataka, India.
Department of Pharmaceutical Chemistry 2, Manipal College of Pharmaceutical Sciences, Manipal - 576104, Karnataka, India.
ABSTRACT: Heterocyclic systems are a part of a large number of drugs and biologically relevant molecules. The chemistry and biological study of heterocyclic compounds have been an interesting field for a long time, and oxazole is one such moiety that has gained attention in recent times due to its increasing importance in the field of medicinal chemistry. Oxazole is a doubly unsaturated five-membered ring having one oxygen atom at position 1 and nitrogen at position 3 separated by a carbon atom in between. The substituted pattern in oxazole derivatives play a vital role in delineating the biological activities like antimicrobial, antifungal, antitubercular, anticancer. The utility of oxazole as intermediates for the synthesis of new chemical entities in medicinal chemistry has been increased in the past few years. Oxazole is an important heterocyclic nucleus having a wide spectrum of biological activities, which drew the attention of researchers around the globe to synthesis various oxazole derivatives and screen them for their various pharmacological activities like antimicrobial activity, antitubercular activity, anticancer activity. 6-(substitutedbenzylidene)-2-methylthiazolo [2, 3-b] oxazol-5(6H)-one were prepared by dissolving 1- hydroxypropan-2-one and KSCN in ethanol and screened for anticancer activity. A series of 6-(substituted benzylidene)-2-methylthiazolo [2, 3-b] oxazol-5(6H)-one O13-24 were tested against anticancer activity. Their activity was evaluated by MTT assay method against cervical HeLa (ME 180) cells. The results of studies indicates that, among the compounds, O13, O14, O15, O16, O17, O21, O23, and O24 displayed significant activity. The purity of the compounds was characterized by means of IR, 1HNMR mass spectral, and elemental analysis. Of these compounds, O14 and O24 showed enhanced activity.
Keywords: |
Oxazole, Anticancer Activity, KSCN, MTT Assay
INTRODUCTION: Heterocyclic chemistry is the branch of science which involves synthesis, properties, and applications of heterocycles. Heterocyclic systems containing mainly nitrogen, sulfur, and oxygen atom 1 constitute a huge set of compounds of biological and medicinal concern.
A massive number of heterocyclic systems, which include mainly five and six-membered compounds, symbolize a different group of molecular scaffolds. All the heterocyclic compounds have a great interest in medicinal and Pharmaceutical chemistry.
Among the heterocyclic compounds, oxazole is a well-known privileged structure in medicinal chemistry 2, having various biological activities. Oxazole is an important pharmacophore in modern drug discovery, and one of the main resources for medicinal research and the nucleus contains a five-membered ring. Oxazole is heterocyclic compounds which contain a five-membered ring and analogs can be suitably modified by the introduction of different aromatic and heterocyclic moieties to exhibit a wide variety of biological activities 3. This nucleus is present in many marketed drugs as well as these nucleus substituted derivatives have long been regarded as possessing principal pharmacophore used in the treatment of various diseases 4. Because of its excellent stability, bioavailability, biological activity it became a prime focus in the medicinal research field.
They exemplify the biological significance and beneficial efficacy of some heterocyclic derivatives used as antiamoebic 5, anthelmintic, anticancer activity 6, antioxidant activity 7 anti-inflammatory 7, anti-microbial activity 8, antibacterial 9-11, antifungal 12 anti-infective agents 13, cytotoxic activity 14, protein-kinase 15, antiviral 16, analgesic and anticonvulsant 17, antiproliferative activity 18, anticancer activity 19. Thus, the learning of chemistry and the biological importance of heterocyclic compounds has been an exciting area of research for an extended occasion.
Current literature has explored the biological consequence of an assortment of structural derivatives of heterocyclic compounds. Cancer is a serious worldwide health threat, killing almost seven million people a year. Human cancer comprises more than 200 different diseases together; they account for about one-fifth of all deaths worldwide. Apart from the already marketed drugs, there are many others being investigated for their promising activity against several malignancies. In particular, anticancer research has been capitalizing on the intrinsic versatility and dynamic core scaffold of these compounds. Nevertheless, as for any other promising anticancer drugs, heterocyclic compounds do not come without shortcomings. It is important to study heterocyclic active compounds and families and their main applications in medicine 20. Carcinoma type of cancer largely manifests in the population than sarcoma 21.
The aim is to develop research interest to develop new potent molecules that act against cancer with slightest side effects.
MATERIALS AND METHODS:
Materials: Synthetic starting material, reagents, and solvents were of analytical reagent grade or of the highest quality commercially available and were purchased from Aldrich Chemical Co., Merck Chemical Co., and were dried when necessary. The melting points were taken in an open capillary tube and are uncorrected. IR spectra were recorded with KBr pellets (ABB Bomem FT-IR spectrometer MB 104 ABB Limited Bengaluru, India). Proton (1H) NMR spectra (Bruker 400 NMR spectrometer Mumbai, India) were recorded with TMS as internal references. Mass spectral data were recorded with a quadrupol mass spectrometer (Shimadzu GC MS QP 5000, Chennai, India), and microanalyses were performed using a vario EL V300 elemental analyzer (Elemental Analysensysteme GmbH Chennai, India). The purity of the compounds was checked by TLC on pre-coated SiO2 gel (HF254, 200 mesh) aluminum plates (E. Merck) using ethyl acetate: benzene (1:3) and visualized in UV chamber. IR, 1H-NMR, mass spectral data and elemental analyses were consistent with the assigned structures.
Synthetic Procedure: Hydroxypropan-2-one (1.00 g,) and KSCN (1.07 g,) were dissolved in ethanol (30 mL). After cooling at -5 °C, 12M aqueous HCl (1.10 mL,) was carefully added, and the mixture was stirred under reflux for 24 h, and then it was cooled by using crushed ice. After extraction with ethyl acetate (3 × 25 mL), the combined organic phase was successively washed with saturated aqueous sodium bicarbonate, water, and finally, it was dried using Magnesium sulphate. After filtration and concentration under reduced pressure, the residue was collected to afford compound 1. A mixture of 5-methyloxazole-2(3H)-thione 1 chloro-acetic acid (0.006 mol), sodium acetate anhydrous (6 g) in glacial acetic acid and acetic acid anhydride (120 mL, 3:1) was refluxed for 12 min.
Further, an equimolecular amount (0.006 mol) of the appropriate aromatic aldehydes was added. The reaction mixture was refluxed for 2 h, allowed to cool and was poured into crushed ice water; the formed precipitate was filtered off, dried, and recrystallized by using ethanol to give the corresponding 6-(substitutedbenzylidene)-2- methylthiazolo [2,3-b]oxazol-5(6H)-one O13-24.
Anticancer Screening:
MTT Test: The MTT assay was performed by using human cervical carcinoma cell line (HeLa), and it was obtained from the National Cancer Institute. The cells were cultured under the standard conditions in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) 100 IU/ml of Penicillin and 100 mg/ml of Streptomycin. The cells were incubated at 37 °C under 5% CO for 24 h. The anticancer activity on HeLa cell line by sulforhodamine B assay as earlier reported method 22. After the 1 × 04 cells were seeded into a 96-well plate and maintained in the DMEM medium at 37 °C under 5% CO for 24 h, the cells were treated with the synthesized compounds at various concentrations for 24 h. Cisplatin was used as a standard. The absorbance was measured at 570 nm by a microplate reader. The percentages of the growth inhibition (G) were determined by the following method 23, 24.
Scheme for- 6-benzylidene-2-methylthiazolo[2,3-b]oxazol-5(6H)-one:
SCHEME 1: Scheme for- 6-benzylidene-2-methylthiazolo[2,3-b]oxazol-5(6H)-one
Spectral Data of the Synthesized Data:
6-(2-aminobenzylidene)-2-methylthiazolo[2, 3-b] oxazol-5(6H)-one (O13): The compound was obtained as a solid; Yield: 61%; m.p. 162-164 °C.
IR cm-1: 3447 (1oN-H), 3027 (Ar-CH), 2911 (Alkane-CH), 1736 (C=O), 1338 (C-N), 1277 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.84 (s, 1H; Alkene-CH), 6.79-7.49 (m, 4H; Ar-H), 6.36 (s, 2H; Oxa-zole-CH), 5.47 (s, 2H; -NH2), 2.27 (s, 3H; -CH3).
EI-MS m/z (M+): 260 (calcd for C13H12N2O2S; 260.31).
Anal. calcd for C13H12N2O2S; C, 59.98; H, 4.65; N, 10.76; found: C, 59.94; H, 4.63; N, 10.72.
6- (3-aminobenzylidene)-2-methylthiazolo[2,3-b] oxazol-5(6H)-one (O14): The compound was obtained as a solid; Yield: 61%; m.p. 169-171 °C.
IR cm-1: 3350 (1oN-H), 3034 (Ar-CH), 2918 (Alkane-CH), 1662 (C=O), 1352 (C-N), 1294 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.81 (s, 1H; Alkene-CH), 6.72-7.41 (m, 4H; Ar-H), 6.31 (s, 2H; Oxazole-CH), 5.43 (s, 2H; -NH2), 2.23 (s, 3H; -CH3).
EI-MS m/z (M+): 260 (calcd for C13H12N2O2S; 260.31).
Anal. calcd for C13H12N2O2S; C, 59.98; H, 4.65; N, 10.76; found: C, 59.95; H, 4.64; N, 10.73.
6- (4-aminobenzylidene)-2-methylthiazolo[2,3-b] oxazol-5(6H)-one (O15): The compound was obtained as a solid; Yield: 61%; m.p.197-199 °C.
IR cm-1: 3446 (1oN-H), 3025 (Ar-CH), 2916 (Alkane-CH), 1735 (C=O), 1336 (C-N), 1275 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.74 (s, 1H; Alkene-CH), 6.74-7.45 (m, 4H; Ar-H), 6.34 (s, 2H; Oxazole-CH), 5.45 (s, 2H; -NH2), 2.24 (s, 3H; -CH3).
EI-MS m/z (M+): 260 (calcd for C13H12N2O2S; 260.31).
Anal. calcd for C13H12N2O2S; C, 59.98; H, 4.65; N, 10.76; found: C, 59.97; H, 4.65; N, 10.74.
6-(2-methylbenzylidene)-2-methylthiazolo[2,3-b] oxazol-5(6H)-one (O16): The compound was obtained as a solid; Yield: 64%; m.p.142-144 °C.
IR cm-1: 3027 (Ar-CH), 2911 (Alkane-CH), 1725 (C=O), 1331 (C-N), 1275 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.77 (s, 1H; Alkene-CH), 6.71-7.42 (m, 4H; Ar-H), 6.31 (s, 2H; Oxazole-CH), 2.29 (s, 6H; -CH3).
EI-MS m/z (M+): 259 (calcd for C14H13NO2S; 259.32).
Anal. calcd for C14H13NO2S; C, 64.84; H, 5.05; N, 5.40; found: C, 64.88; H, 5.05; N, 5.43.
6-(3-methylbenzylidene)-2-methylthiazolo[2,3-b] oxazol-5(6H)-one (O17): The compound was obtained as a solid; Yield: 61%; m.p. 177-179 °C.
IR cm-1: 3122 (Ar-CH), 3012 (Alkane-CH), 1654 (C=O), 1351 (C-N), 1250 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.79 (s, 1H; Alkene-CH), 6.71-7.41 (m, 4H; Ar-H), 6.31 (s, 2H; Oxazole-CH), 2.29 (s, 6H; -CH3).
EI-MS m/z (M+): 259 (calcd for C14H13NO2S; 259.32).
Anal. calcd for C14H13NO2S; C, 64.84; H, 5.05; N, 5.40; Found: C, 64.86; H, 5.06; N, 5.42.
6-(4-methylbenzylidene)-2-methylthiazolo[2,3-b] oxazol-5(6H)-one (O18): The compound was obtained as a solid; Yield: 69%; m.p. 182-184 °C.
IR cm-1: 3029 (Ar-CH), 2919 (Alkane-CH), 1737 (C=O), 1334 (C-N), 1271 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.87 (s, 1H; Alkene-CH), 6.94-7.47 (m, 4H; Ar-H), 6.54 (s, 2H; Oxazole-CH), 2.14 (s, 6H; -CH3).
EI-MS m/z (M+): 259 (calcd for C14H13NO2S; 259.32).
Anal. calcd for C14H13NO2S; C, 64.84; H, 5.05; N, 5.40; found: C, 64.82; H, 5.02; N, 5.46.
6- (2-methoxybenzylidene)-2-methylthiazolo[2,3-b]oxazol-5(6H)-one (O19): The compound was obtained as a solid; Yield: 65%; m.p.147-149 °C
IR cm-1: 3120 (Ar-CH), 2933 (Alkane-CH), 1726 (C=O), 1342 (C-N), 1271 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.71 (s, 1H; Alkene-CH), 6.62-7.51 (m, 4H; Ar-H), 6.56 (s, 2H; Oxazole-CH), 4.29 (s, 3H; -OCH3), 2.10 (s, 3H; -CH3).
EI-MS m/z (M+): 275 (calcd for C14H13NO3S; 275.32).
Anal. calcd for C14H13NO3S; C, 61.07; H, 4.76; N, 5.09; found: C, 61.05; H, 4.75; N, 5.07.
6- (3-methoxybenzylidene)-2-methylthiazolo[2,3-b]oxazol-5(6H)-one (O20): The compound was obtained as a solid; Yield: 66%; m.p. 151-153 °C.
IR cm-1: 3011 (Ar-CH), 2911 (Alkane-CH), 1722 (C=O), 1333 (C-N), 1276 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.74 (s, 1H; Alkene-CH), 6.64-7.57 (m, 4H; Ar-H), 6.54 (s, 2H; Oxa-zole-CH), 4.27 (s, 3H; -OCH3), 2.17 (s, 3H; -CH3).
EI-MS m/z (M+): 275 (calcd for C14H13NO3S; 275.32).
Anal. calcd for C14H13NO3S; C, 61.07; H, 4.76; N, 5.09; found: C, 61.07; H, 4.73; N, 5.05.
6- (4-methoxybenzylidene)-2-methylthiazolo[2,3-b]oxazol-5(6H)-one (O21): The compound was obtained as a solid; Yield: 71%; m.p. 167-169 °C.
IR cm-1: 3029 (Ar-CH), 2917 (Alkane-CH), 1739 (C=O), 1331 (C-N), 1277 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 7.84 (s, 1H; Alkene-CH), 6.69-7.59 (m, 4H; Ar-H), 6.59 (s, 2H; Oxa-zole-CH), 4.34 (s, 3H; -OCH3), 2.29 (s, 3H; -CH3).
EI-MS m/z (M+): 275 (calcd for C14H13NO3S; 275.32).
Anal. calcd for C14H13NO3S; C, 61.07; H, 4.76; N, 5.09; found: C, 61.04; H, 4.71; N, 5.01.
6- (2-hydroxybenzylidene)-2-methylthiazolo[2,3-b]oxazol-5(6H)-one (O22): The compound was obtained as a solid; Yield: 64%; m.p. 171-173 °C.
IR cm-1: 3634 (Phenolic O-H), 3013 (Ar-CH), 2921 (Alkane-CH), 1726 (C=O), 1329 (C-N), 1262 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 8.42 (s, 1H; Phenolic-OH), 7.61 (s, 1H; Alkene-CH), 6.47-7.25 (m, 4H; Ar-H), 6.32 (s, 2H; Oxazole-CH), 2.27 (s, 3H; -CH3).
EI-MS m/z (M+): 261 (calcd for C13H11NO3S; 261.3).
Anal. calcd for C13H11NO3S; C, 59.76; H, 4.24; N, 5.36; found: C, 59.73; H, 4.22; N, 5.33.
6- (3-hydroxybenzylidene)-2-methylthiazolo[2,3-b]oxazol-5(6H)-one (O23): The compound was obtained as a solid; Yield: 71%; m.p. 162-164 °C.
IR cm-1: 3385 (Phenolic O-H), 3176 (Ar-CH), 2901 (Alkane-CH), 1668 (C=O), 1321 (C-N), 1227 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 8.11 (s, 1H; Phenolic-OH), 7.32 (s, 1H; Alkene-CH), 6.61-7.27 (m, 4H; Ar-H), 6.37 (s, 2H; Oxazole-CH), 2.21 (s, 3H; -CH3).
EI-MS m/z (M+): 261 (calcd for C13H11NO3S; 261.3).
Anal. calcd for C13H11NO3S; C, 59.76; H, 4.24; N, 5.36; found: C, 59.76; H, 4.24; N, 5.34.
6-(4-hydroxybenzylidene)-2-methylthiazolo[2, 3-b]oxazol-5(6H)-one (O24): The compound was obtained as a solid; Yield: 64%; m.p. 171-173 °C.
IR cm-1: 3612 (Phenolic O-H), 3015 (Ar-CH), 2927 (Alkane-CH), 1729(C=O), 1321 (C-N), 1263 (Ether C-O-C).
1H NMR (CDCl3) δ (ppm): 8.31 (s, 1H; Phenolic-OH), 7.66 (s, 1H; Alkene-CH), 6.41-7.21 (m, 4H; Ar-H), 6.33 (s, 2H; Oxazole-CH), 2.24 (s, 3H; -CH3).
EI-MS m/z (M+): 261 (calcd for C13H11NO3S; 261.3).
Anal. calcd for C13H11NO3S; C, 59.76; H, 4.24; N, 5.36; found: C, 59.75; H, 4.23; N, 5.35.
RESULTS AND DISCUSSION:
Chemistry: The chemical structure of chlorogenic acid was confirmed by IR, 1H-NMR, 13C-NMR, and mass spectroscopy. In 6-(substituted-benzylidene)-2-methylthiazolo[2,3-b]oxazol-5(6H) one O1-12, Ar- CH stretching band appears in the range of 3150-3050 cm-1 and the appearance of a strong intensity band in the IR spectra of Alkane-CH in the range of 3000-2850 cm-1. The appearance of 1740-1705, 1300-1000 cm-1 range was attributable to ester C=O, Ether C-O-CH, respectively.
Anticancer Screening:
Results of anti-cancer screening of 6-benzylidene-2- methylthiazolo [2,3-b] oxazol-5(6h)-one:
TABLE 1: ANTI-CANCER SCREENING OF THE SYNTHESIZED COMPOUNDS O13-O24
Compound | Cell line | Compound Concentration (μ mol L-1) | IC 50 | |||
% Growth Inhibition | ||||||
5 | 12.5 | 25 | 40 | |||
O13 |
HeLa
|
51.55 | 69.43 | 79.63 | 90.16 | 19.46 |
O14 | 62.22 | 79.36 | 89.11 | 97.18 | 13.42 | |
O15 | 52.25 | 74.23 | 86.32 | 92.42 | 19.23 | |
O16 | 56.25 | 78.32 | 89.42 | 96.42 | 19.15 | |
O17 | 54.2 | 66.42 | 78.31 | 89.13 | 16.34 | |
O18 | 9.4 | 12.12 | 18.32 | 19.42 | >100 | |
O19 | 8.22 | 9.34 | 14.21 | 16.42 | >100 | |
O20 | 6.43 | 8.21 | 12.51 | 15.22 | >100 | |
O21 | 44.12 | 64.11 | 74.26 | 88.42 | 19.46 | |
O22 | 2.41 | 4.12 | 6.21 | 10.24 | >100 | |
O23 | 51.22 | 71.36 | 83.34 | 89.41 | 15.12 | |
O24 | 54.11 | 62.12 | 71.37 | 90.28 | 11.37 | |
Cisplatin | 69.21 | 79.42 | 86.32 | 99.31 | 24.13 |
Anticancer Screening:
Discussion of Anti-cancer Screening of– 6-benzylidene- 2- methylthiazolo [2, 3-b] oxazol-5(6h)-one: The title compounds 6-(substituted benzylidene)- 2- methylthiazolo [2, 3-b] oxazol-5(6H)-one O13- 24 were screened for their Anti-cancer activity against cervical HeLa (ME 180) cells and this activity is done at the Karnataka college of pharmacy by using the MTT assay which was determined by previously reported methods. The different concentrations (5, 12.5, 25, and 40 µmol L-1) were used to test Anti-cancer activity. The concentration required for 50% inhibition of cell viability (IC) was calculated and compared with the reference drug Cisplatin, and the results are given in Table 1.
The data from Table 1 reveals that the compounds O18, O19, O20, and O22 were inactive at all the concentrations used. The compounds O13, O14, O15, O16, O17, O21, O23, and O24 show significant activity towards the HeLa (ME 180) cell lines and also showed equipotent activity with the control Cisplatin. Of these, the compounds O24, O14, which has a para-substituted hydroxyl group and meta substituted amino group, showed enhanced activity. It was considered the most potent analog and found to have significant Anti- cancer activity. It is also observed that the derivatives having the hydroxyl group at either the meta or the para positions were active, while, those derivatives having the methyl group at either of these positions were inactive.
The result revealed that substituted compounds of benzylidene ring, except the methyl-substituted compound, showed significant anticancer activity.
CONCLUSION: The compounds O24, O14, which has a para-substituted hydroxyl group and meta substituted amino group, were considered as the most potent analog and found to have significant anticancer activity.
The synthesized analogs will generate a good impact to the chemists and research scholars for further investigation in this field of oxazole and selectively being the influence of electronic effects as well as the change in basic nucleus possessing significant activity.
ACKNOWLEDGEMENT: Authors are very much thankful to Karnataka College of Pharmacy, Bangalore, India, for constant help and support.
CONFLICTS OF INTEREST: The authors declare that there is no conflicts of interest.
REFERENCES:
- Joshi S, Bisht AS and Juyal D: Systematic scientific study of 1, 3-oxazole derivatives as a useful lead for pharmaceuticals: a review. The Pharma Innovation 2017; 6(1): 109-17.
- Zhang H, Zhao Z and Zhou C: Recent advance in oxazole-based medicinal chemistry. Eur J Med Chem 2018; 144: 444-92.
- Rawat BS and Shukla SK: Synthesis and evaluation of some new thiazole/oxazole derivatives for their biological activities. World Journal of Pharmacy and Pharmaceutical Sciences 2016; 5(8): 1473-82.
- Yerragunta V, Suman D, Swamy K, Anusha V, Patil P and Naresh M: Pyrazole and its biological activity. PharmaTutor 2014; 2(1): 40-8.
- Kakkar S and Narasimhan B: A comprehensive review on biological activities of oxazole derivatives. BMC Chemistry 2019; 13(1): 16.
- Siddiqui SM, Salahuddin A and Azam A: Synthesis, characterization and antiamoebic activity of some hydrazone and azole derivatives bearing pyridyl moiety as a promising heterocyclic scaffold. Eur J Med Chem 2012; 49: 411-6.
- Mathew B, Hobrath JV, Connelly MC, Guy RK and Reynolds RC: Oxazole and thiazoleanalogs of sulindac for cancer prevention. Future Medicinal Chemistry 2018; 10(7): 743-53.
- Yatam S, Jadav SS, Gundla R, Gundla KP, Reddy GM, Ahsan MJ and Chimakurthy J: Design, synthesis and biological evaluation of 2 (((5-aryl-1, 2, 4-oxadiazol-3-yl) methyl) thio) benzo [d] oxazoles: new anti-inflammatory and antioxidant agents. Chemistry Select 2018; 3(37): 10305-10.
- Wales SM, Hammer KA, Somphol K, Kemker I and Schroder DC: Synthesis and antimicrobial activity of binaphthyl-based, functionalized oxazole and thiazole peptidomimetics. Organic & Biomolecular Chemistry 2015; 13(44): 10813-24.
- Boukhssas S, Aouine Y, Faraj H, Alami A, El Hallaoui A and Bekkari H: Synthesis, characterization, and antibacterial activity of diethyl 1-((4-methyl-2-phenyl-4, 5-dihydrooxazol-4-yl) methyl)-1H-1, 2, 3-triazole-4, 5-dicarboxylate. Journal of Chemistry 2017; 1-6.
- Alothman OY: Synthesis and antimicrobial activities of some new heterocyclic compounds based on 6-chloropyridazine-3 (2H)-thione. Journal of Chemistry 2013.
- Azab ME, Youssef MM and El-Bordany EA: Synthesis and antibacterial evaluation of novel heterocyclic compounds containing a sulfonamido moiety. Molecules 2013; 18(1): 832-44.
- Pitucha M, Pachuta-Stec A and Kaczor AA: New five-membered ring heterocyclic compounds with antibacterial and antifungal activity. Microbial pathogens and strategies for combating them. Science, Technology and Education 2013; 1: 562-73.
- Kumar S, Bawa S, Drabu S, Kumar R and Gupta H: Biological activities of pyrazoline derivatives-A recent development. Recent patents on Anti-Infective Drug Discovery 2009; 4(3): 154-63.
- Puthiyapurayil P, Poojary B, Chikkanna C and Buridipad SK: Design, synthesis and biological evaluation of a novel series of 1, 3, 4-oxadiazole bearing N-methyl-4-(trifluoromethyl) phenyl pyrazole moiety as cytotoxic agents. European Journal of Medicinal Chemistry 2012; 53: 203-10.
- Das J, Pany S, Panchal S, Majhi A and Rahman GM: Binding of isoxazole and pyrazole derivatives of curcumin with the activator binding domain of novel protein kinase C. Bioorganic & Medicinal Chemistry 2011; 19(21): 6196-202.
- Salem MS, Sakr SI, El‐Senousy WM and Madkour HM: Synthesis, antibacterial, and antiviral evaluation of new heterocycles containing the pyridine moiety. Archiv der Pharmazie 2013; 346(10): 766-73.
- El-Sawy ER, Ebaid MS, Abo-Salem HM, Al-Sehemi AG and Mandour AH: Synthesis, anti-inflammatory, analgesic and anticonvulsant activities of some new 4, 6-dimethoxy-5-(heterocycles) benzofuran starting from naturally occurring visnagin. Arabian Journal of Chemistry 2014; 7(6): 914-23.
- Chen Y, Yu K, Tan NY, Qiu RH, Liu W, Luo NL, Tong L, Au CT, Luo ZQ and Yin SF: Synthesis, characterization and anti-proliferative activity of heterocyclic hypervalent organoantimony compounds. European Journal of Medicinal Chemistry 2014; 79: 391-8.
- El-Sawy ER, Mandour AH, El-Hallouty SM, Shaker KH and Abo-Salem HM: Synthesis, antimicrobial and anticancer activities of some new N-methylsulphonyl and N-benzenesulphonyl-3-indolyl heterocycles: 1st Cancer Update. Arabian Journal of Chemistry 2013; 6(1): 67-78.
- Martins P, Jesus J, Santos S, Raposo LR and Roma-Rodrigues C: Heterocyclic anticancer compounds: recent advances and the paradigm shift towards the use of nanomedicine’s tool box. Molecules 2015; 20(9): 16852-91.
- Nakao S, Mabuchi M, Shimizu T, Itoh Y and Takeuchi Y: Design and synthesis of prostate cancer antigen-1 (PCA-1/ALKBH3) inhibitors as anti-prostate cancer drugs. Bioorganic & Medicinal Chemistry Letters 2014; 24(4): 1071-4.
- Manosroi J, Wilairat R and Manosroi A: Anti-Proliferative activity of extracts from thai Plantsin guttiferae and Schisandraceae families on human cancer cell lines. Pharmaceutical Biology 2007; 45(3): 255-8.
- Papazisis KT, Geromichalos GD, Dimitriadis KA and Kortsaris AH: Optimization of the sulforhodamine B colorimetric assay. Journal of Immunological Methods 1997; 208(2): 151-8.
- Florento L, Matias R, Tuano E, Santiago K, dela Cruz F and Tuazon A: Comparison of cytotoxic activity of anticancer drugs against various human tumor cell lines using in vitro cell-based approach. International Journal of biomedical science. IJBS 2012; 8(1): 76.
How to cite this article:
Kini DD and Mathews JE: Synthesis and biological evaluation of novel 6-(substituted benzylidene)-2-methylthiazolo [2,3-b] oxazol-5(6h)-one as potential anticancer agents. Int J Pharm Sci & Res 2020; 11(5): 2199-05. doi: 10.13040/IJPSR.0975-8232.11(5).2199-05.
All © 2013 are reserved by the International Journal of Pharmaceutical Sciences and Research. This Journal licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
Article Information
26
2199-2205
495
874
English
IJPSR
D. D. Kini * and J. E. Mathews
Department of Pharmaceutical Chemistry, Karnataka College of Pharmacy, Bangalore, Karnataka, India.
deepthikini@gmail.com
18 June 2019
19 February 2020
02 April 2020
10.13040/IJPSR.0975-8232.11(5).2199-05
01 May 2020