SYNTHESIS, CHARACTERIZATION AND ANTICANCER ACTIVITY OF PIPERAZINE AMIDE DERIVATIVE

Piperazine is a nitrogen-containing heterocyclic compound and has fascinating applications in drug discovery and development. In the present study, novel 4-(benzo[1,3]dioxol-5-ylmethyl) piperazine amide derivative was prepared by acid amine coupling of 1-piperonyl piperazine derivative and benzoic acid. Synthesized compound was characterized by IR, NMR and mass spectral studies. Further, in-vitro cytotoxic effects were carried out in MTT in various human cell lines HeLa, MCF7, MDA-MB-231, HCT116, and HT29. Out of these diverse cell lines, compound 3 showed promising cytotoxic effects on MDA-MB-231 with the IC₅₀ estimation of 11.3 µM. The surface morphology by Colonogenic assay and in-vitro cell migration assay measures better movement on MDA-MB-231 in compound 3. Further, apoptotic-related examination under AO/Eb staining, commet assay, and cell cycle investigation exhibited that compound 3 demonstrated significant activities on MDA-MB-231 by activates apoptosis and blocking cell cycle moment in the G0/G1 stage. All the more altogether, staining the cells in the meantime with Annexin V-FITC/PI. The synthesized compound 3 indicated tremens-dous repressing capacities in cell cycle and activates apoptosis because of the structural morphology and heteroatom present in the molecule.