SYNTHESIS, CHARACTERIZATION AND IN-VITRO ANTICANCER SCREENING OF VITIS VINIFERA SEED ETHANOLIC EXTRACT LOADED CHITOSAN NANOPARTICLES IN H-29 COLON CANCER CELL LINEAbstract
Nanotechnology can be applied to deliver and protect antioxidants in order to control the oxidative stress phenomena in several chronic pathologies. Chitosan (CS) and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible, and biodegradable, and, hence, suitable for various biomedical applications, such as drug delivery gene therapy and tissue engineering. Vitis vinifera (VV) seeds are the major chemotherapeutic agent with antioxidant, anti-inflammatory, antiproliferative, anticancer, and antimicrobial effects. However, the potential of these leaves as a chemotherapeutic agent is limited by their hydrophobicity and poor bioavailability. The chitosan tripolyphosphate (CS/TPP) nanoparticles have been used in this study as an alternative to encapsulate bioactive flavonoids from ethanolic extract of Vitis vinifera seeds. The Vitis vinifera seeds extract-loaded CS (CS-VVE) nanoparticles were characterized using FT-IR. The Zeta sizer and zeta potential confirmed that the particle has 216nm and 50.3mV. This study also found that the CS-VVE NPs shown anticancer activity in human colon cancer cell lines should be explored further for Cytotoxicity studies using MTT assay and tested drug-induced apoptotic process in the cell lines confirmed by the Dual staining. The CS-VVE nanoparticles showed specific toxicity towards cancer cells and non-toxicity to normal cells. It is suggested that the chitosan nanoparticles fabricated in our study may provide a suitable alternative to traditional adjuvant systems. Hence, it was observed from the present investigation that the anticancer activity of Vitis vinifera seed extract loaded chitosan nanoparticles provided a platform for treating cancer with biopolymer nanomaterials.