SYNTHESIS OF 3- (1 – BENZYL – 1H – BENZO [D] IMIDAZOL – 2 – L AMINO) – 2 – (3 – ARYL – 1- PHENYL – 1H – PYRAZOL – 4 – YL) THIAZOLIDIN – 4- ONES AND THEIR ANTIMICROBIAL ACTIVITIES
HTML Full TextSYNTHESIS OF 3- (1 – BENZYL - 1H - BENZO [D] IMIDAZOL – 2 - L AMINO) – 2 - (3 – ARYL - 1- PHENYL - 1H - PYRAZOL - 4 - YL) THIAZOLIDIN - 4- ONES AND THEIR ANTIMICROBIAL ACTIVITIES
Sharanabasappa B. Patil and Naganna M. Goudgaon*
Department of Studies and Research in Chemistry, Gulbarga University, Gulbarga (Karnataka), India
ABSTRACT
Reaction of 1- (1- benzyl- 1H- benzo [d] imidazol- 2- yl) hydrazine (1) with 3-aryl-1-phenyl-1H-pyrazole-4-carbaldehydes (2a-f) in ethanol yielded the corresponding Schiff bases (3a-f). Further, cyclization of compounds 3a- f with thioglycollic acid in benzene in presence of anhydrous ZnCl2 furnished desired novel compounds 3- (1 – Benzyl - 1h - Benzo [D] Imidazol – 2 - L Amino) – 2 - (3 – Aryl - 1- Phenyl - 1h - Pyrazol - 4 - Yl) Thiazolidin - 4- Ones (4a- f) in 41-65% yield. All the synthesized compounds were screened for antibacterial and antifungal activities. Among the synthesized compounds 3b, 3d, 4a and 4f have shown good activity against bacteria P. aeruginosa, S. aureus and P. vulgaris, where as other compounds have shown moderate to poor activity against all the organisms. The compounds 3a and 3c exhibited good activity against fungal strains A. niger and A. flavus. All remaining synthesized compounds exhibited moderate to poor activity against all the organisms. The structures of synthesized compounds have been established by spectral studies and elemental analysis.
Keywords:
1- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl) hydrazine, Pyrazoles, Schiff base, Thiazolidinones, Antimicrobial activity |
INTRODUCTION: Benzimidazoles constitute an important group of heterocyclic compounds and have been shown to exhibit wide range of pharmacological activities such as antifungal, antibacterial, antiparasitic and anthelmintic 1-3. Some of the benzimidazole analogs like thiabendazole, mebendazloe and albebdazole are widely used as anthelmintic drugs 4. In addition, N1 and C2- subtituted benzimidazoles and their derivatives have been found to be potent biologically active compounds as well. Further, N1-subtituted benzimidazoles have exhibited anti-microbial 5 and also antiviral activity 6 against human cytomegalovirus and herpes simplex virus type-1. The biological activities of these compounds depend upon the substitution on the benzimidazole at the N-1 or C-2 position. In addition, pyrazole and thiazolidinone derivatives are also known to exhibit a wide range of biological activities such as anti-hyperglycaemic, analgesics, anti-inflammatory, antipyretic, antibacterial and sedative-hypnotics 7-11. In continuation of our research work on benzimidazoles 12-14 and pyrazoles 15, we report here the synthesis and antimicrobial activity of some novel 3 (1- benzyl- 1H- benzo [d] imidazole- 2-yl amino)- 2- (3- aryl- 1- phenyl- 1H- pyrazole- 4-yl) thiazolidine- 4- ones.
EXPERIMENTAL:
Chemistry: Melting points were recorded by using Thomas-Hoover melting point apparatus and were uncorrected. IR spectra in KBr disc were recorded on Perkin- Elmer- Spectrum- one FTIR spectrophotometer (νmax in cm-1) and 1H NMR in DMSO-d6 on amx 400 MHz spectrophotometer using TMS as internal standard (chemical shift in δ or ppm). Mass spectra were recorded on a JEOL SX 102 Mass spectrometer using Argon/Xenon (6kv, 10 mA) as the FAB gas. Purity of the compounds was checked by TLC using silica gel ‘G’ plates obtained from Whatman Inc, and a fluorescent indicator. 3- aryl- 1- phenyl- 1H- pyrazole- 4-carbaldehydes were prepared by known literature method 16.
General procedure for the synthesis of Schiff bases (3a- f): A mixture 1- (1- benzyl- 1H-benzo [d] imidazol- 2- yl) hydrazine (1) (0.01 mol) and 3- aryl- 1- phenyl- 1H- pyrazole- 4- carbaldehydes (2a-f) (0.01 mol) in ethanol (20 ml) containing 3-4 drops of conc. HCl was refluxed on a water bath for 8 hours. Concentrated and the residue was recrystalized from methanol yielded desired compounds 3a-f.
1- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl)- 2-((1, 3- diphenyl- 1H- pyrazol- 4-yl) methylene) hydrazine (3a): Yield 1.5 g (60%). IR (KBr): 3253, 3182 (NH). 1H NMR: 10.0 (s, 1H, N1H), 7.9-7.6 ( 2H 1 N=CH, C5H of pyrazole), 7.5-7.0 (m, 19H, ArH), 5.2 (s, 2H, methylene proton). Mass (m/z) = 468 (M+, 10%). Anal. Calcd for C30H24N6 : C, 76.90; H, 5.16; N, 17.94. Found: C, 75.90; H, 5.15; N, 16.94.
1- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl)- 2- ((3- (4- nitrophenyl)- 1- phenyl- 1H- pyrazol- 4-yl) methylene) hydrazine (3b): Yield 1.2 g (55%). IR (KBr): 3139, 3121 (NH). 1H NMR: 10.2 (s, 1H, N1H), 7.8-7.6 ( 2H 1 N=CH, C5H of pyrazole), 7.5-7.0 (m, 18H, ArH), 5.2 (s, 2H, methylene proton). Mass (m/z) = 513 (M+, 10%). Anal. Calcd for C30H23N7O2 : C, 70.16; H, 4.51; N, 19.09. Found: C, 69.16; H, 4.50; N, 19.08.
1- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl)- 2- ((1-phenyl- 3- p- tolyl- 1H- pyrazol- 4-yl) methylene) hydrazine (3c): Yield 1.2 g (65%). IR (KBr): 3413, 3123 (NH). 1H NMR: 10.1 (s, 1H, N1H), 7.8-7.6 ( 2H, 1 N=CH, C5H of pyrazole), 7.5-7.0 (m, 18H, ArH), 5.2 (s, 2H, methylene proton), 2.2(s, 3H, CH3). Mass (m/z) = 482 (M+, 10%). Anal. Calcd for C31H26N6: C, 77.15; H, 5.43; N, 17.41. Found: C, 77.14; H, 5.42; N, 17.40.
1- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl)- 2- ((3-(2, 4- dimethoxyphenyl)- 1- phenyl- 1H- pyrazol- 4-yl) methylene)hydrazine (3d): Yield 1.4 g (45%). IR (KBr): 3123, 3113 (NH). 1H NMR: 9.9 (s, 1H, N1H), 8.0-7.6( 2H, 1 N=CH, C5H of pyrazole), 7.5-7.2 (m, 17H, ArH), 5.3 (s, 2H, methylene proton), 3.8 (s, 6H, OCH3). Mass (m/z) = 528 (M+, 15%). Anal. Calcd for C32H28N6O2: C, 72.71; H, 5.34; N, 15.90. Found: C, 72.70; H, 5.32; N, 15.89.
1- (1- Benzyl- 1H- Benzo [d] imidazol- 2- yl)- 2- ((3-(2, 5- dimethoxyphenyl)- 1- phenyl- 1H- pyrazol- 4-yl) methylene) hydrazine (3e): Yield 1.5 g (50%). IR (KBr): 3213, 3113 (NH). 1H NMR: 10.0 (s, 1H, N1H), 8.0-7.6 ( 2H, 1 N=CH, C5H of pyrazole), 7.5-7.2 (m, 17H, ArH), 5.2 (s, 2H, methylene proton), 3.8 (s, 6H, OCH3). Mass (m/z) = 528 (M+, 10%). Anal. Calcd for C32H28N6O2: C, 72.71; H, 5.34; N, 15.90. Found: C, 72.69; H, 5.31; N, 15.88.
3- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl amino)- 2- (3- (2- hydroxyphenyl)- 1- phenyl- 1H-pyrazol- 4- yl) methylene) hydrazine (3f): Yield 1.2 g (55%). IR (KBr): 3135, 3123 (NH). 1H NMR: 10.8 (s, 1H, N1H), 8.0 (s, 1H, OH), 7.8-7.6 (2H, 1 N=CH, C5H of pyrazole), 7.0-7.5 (m, 18H, ArH). Mass (m/z) = 484 (M+, 20%). Anal. Calcd for C30H24N6O: C, 74.36; H, 4.99; N, 17.34. Found: C, 74.35; H, 4.97; N, 17.32;
General procedure for the synthesis of 3- (1-Benzyl- 1H- benzo [d] imidazol- 2- yl amino)- 2- (3-aryl-1-phenyl- 1H- pyrazol- 4- yl) thiazolidine- 4- one (4a- f): A mixture of Schiff base (0.001 mol), thioglycollic acid (0.001 mol) and a pinch of ZnCl2 in 10 ml of benzene were refluxed for 24 hours. Concentrated the reaction product under reduced pressure yielded semisolid. It was then neutralized with NaHCO3 (10%). Separated solid was filtered, dried and recrystallized from ethanol gave targeted compounds 4a-f.
3- (1 -Benzyl- 1H- benzo [d] imidazol- 2- yl amino)- 2- (1, 3- diphenyl- 1H- pyrazol- 4- yl) thiazolidin- 4- one (4a): Yield 0.4 g (45%). IR (KBr): 3172, 3123 (NH). 1676 C=O. 1H NMR: 10.0 (s, 1H, N1H), 7.8-7.2 (m, 20H, 19ArH, C5H of pyrazole), 5.6 (s, CH of thiazolidinone), 5.2 (s, 2H, methylene proton), 3.2 (d, CH2 of thiazolidinone). Mass (m/z) = 542 (M+, 10%). Anal. Calcd for C32H26N6OS C, 70.83; H, 4.83; N, 15.49. Found: C, 70.82; H, 4.82; N, 15.48.
3- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl amino)-2- (3- (4- nitrophenyl)- 1-phenyl- 1H- pyrazol- 4- yl) thiazolidin- 4- one (4b): Yield 0.24 g (55%). IR (KBr): 3235, 3123 (NH). 1H NMR: 10.0 (s, 1H, N1H), 8.2-7.2 (m, 19H, 18ArH, C5H of pyrazole), 5.6 (s, CH of thiazolidinone), 5.2 (s, 2H, methylene proton), 3.2 (d, CH2 of thiazolidinone). Mass (m/z) = 587 (M+, 15%). Anal. Calcd for C32H25N7O3S: C, 65.40; H, 4.29; N, 16.68. Found: C, 65.39; H, 4.28; N, 16.66.
3- (1- Benzyl - 1H - benzo [d] imidazol- 2- ylamino) - 2 - (1- phenyl- 3- p- tolyl- 1H- pyrazol- 4- yl) thiazolidin- 4- one (4f): Yield 0.16 g (50%). IR (KBr): 3245, 3211 (NH). 1H NMR: 9.9 (s, 1H, N1H), 7.8-7.2 (m, 19H, 18ArH, C5H of pyrazole), 5.6 (s, CH of thiazolidinone), 5.2 (s, 2H, methylene proton), 3.2 (d, CH2 of thiazolidinone), 2.2 (s, 3H, CH3). Mass (m/z) = 556 (M+, 15%). Anal. Calcd for C33H28N6OS: C, 71.20; H, 5.07; N, 15.10. Found: C, 71.19; H, 5.08; N, 15.08.
3- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl amino)- 2- (3- (2, 4- dimethoxyphenyl)- 1- phenyl- 1H-pyrazol- 4- yl) thiazolidin- 4- one (4c): Yield 0.15 g (65%). IR (KBr): 3245, 3111 (NH). 1H NMR: 10.0 (s, 1H, N1H), 7.9-7.2 (m, 17H, 16ArH, C5H of pyrazole), 5.6 (s, CH of thiazolidinone), 5.2 (s, 2H, methylene proton), 3.2 (d, CH2 of thiazolidinone). Anal. Calcd for C34H30N6O3S: C, 67.76; H, 5.02; N, 13.94. Found: C, 67.75; H, 5.01; N, 13.92.
3- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl amino)- 2- (3- (2, 5- dimethoxyphenyl)- 1- phenyl- 1H-pyrazol- 4- yl) thiazolidin- 4- one (4d): Yield 0.25 g (41%). IR (KBr): 3268, 3112 (NH). 1H NMR: 10.2 (s, 1H, N1H), 7.8-7.2 (m, 17H, 16ArH, C5H of pyrazole), 5.6 (s, CH of thiazolidinone), 5.2 (s, 2H, methylene proton), 3.2 (d, CH2 of thiazolidinone). Anal. Calcd for C34H30N6O3S: C, 67.76; H, 5.02; N, 13.94. Found: C, 67.72; H, 5.02; N, 13.93.
3- (1- Benzyl- 1H- benzo [d] imidazol- 2- yl amino)- 2- (3- (2- hydroxyphenyl)- 1- phenyl- 1H- pyrazol-4- yl) thiazolidin- 4- one (4e): Yield 0.18 g (60%). IR (KBr): 3345, 3211 (NH). 1H NMR: 10.2 (s, 1H, N1H), 7.8-7.2 (m, 19H, 18ArH, C5H of pyrazole), 5.6 (s, CH of thiazolidinone), 5.2 (s, 2H, methylene proton), 3.2 (d, CH2 of thiazolidinone). Mass (m/z) = 558 (M+, 10%). Anal. Calcd for C32H26N6O2S: C, 68.80; H, 4.69; N, 15.04. Found: C, 68.79; H, 4.68; N, 15.02.
RESULTS AND DISCUSSION: The desired novel compounds 4a-f were synthesized in two steps starting from 2- hydrazinobenzimidazole (1) (Scheme- 1). Reaction of 1 with 3- aryl- 1- phenyl- 1H- pyrazole- 4-carbaldehydes (2a-f) in ethanol in the presence of 2-3-drops of conc. HCl under reflux for 8 hours gave the corresponding Schiff bases (3a-f) in 41-65% yield (Table-1). The IR spectrum of compound 3a shows absorptions at 3253 and 3182 cm-1 due to NH and formation of compound 3a was further confirmed by its 1H NMR spectrum shows signals at δ 10.0 (s, 1H, N1H), 7.9-7.6( 2H 1 N=CH, C5H of pyrazole), 7.5-7.0 (m, 19H, ArH), 5.2 (s, 2H, methylene proton ). Mass spectrum of compound 3a shows molecular ion peak at m/z = 468 (M+, 10%).
Cyclization of Schiff bases 3a-f with thioglycollic acid in refluxing benzene in the presence of ZnCl2 for 24 hours furnished 3- (benzo [d] imidazol- 2- yl amino)- 2- (3- aryl- 1- phenyl- 1H- pyrazol- 4-yl) thiazolidin- 4- ones (4a-f). Formation of compound 4a was confirmed by the IR spectra shows absorptions at 3172, 3123 and 1676 cm-1 due to NH and C=O group. 1H NMR spectrum of compound 4a shows signals at δ10.0 (s, 1H, N1H), 7.8-7.2 (m, 20H, 19ArH, C5H of pyrazole), 5.6 (s, CH of thiazolidinone), 5.2 (s, 2H, methylene proton), 3.2 (d, CH2 of thiazolidinone). Further formation of compound 4a was confirmed by mass spectra shows molecular ion peak at m/z = 542 (M+, 10%). Physical constants of all the synthesized compounds are tabulated in Table 1.
TABLE 1: PHYSICAL CONSTANT OF THE SYNTHESIZED COMPOUNDS
COMPOUND | R | YIELD (%) | M. PT. OC |
3a | C6H5 | 60 | 180-182 |
3b | 4-NO2 C6H4 | 55 | 214-216 |
3c | 4-CH3 C6H4 | 65 | 172-174 |
3d | 2,4-(OCH3)2 C6H3 | 45 | 186-188 |
3e | 2,5- (OCH3)2 C6H3 | 50 | 160-162 |
3f | 2-OH C6H4 | 60 | 222-224 |
4a | C6H5 | 45 | 250-252 |
4b | 4-NO2 C6H4 | 55 | Semisolid |
4c | 4-CH3 C6H4 | 50 | Semisolid |
4d | 2,4-(OCH3)2 C6H3 | 65 | Semisolid |
4e | 2,5- (OCH3)2 C6H3 | 41 | Semisolid |
4f | 2-OH C6H4 | 60 | Semisolid |
Antimicrobial Activity: The antimicrobial activities were performed by cup plate method. The sample was dissolved in DMF at the concentration of 1000 µg/ml. Antibacterial activity screened against P. aeruginosa, S. aureus and P. vulgaris. Antifungal activity was carried out against A. niger and A. flavus under aseptic conditions. Gentamycin and fluconazole were used as standard drug for antibacterial and antifungal activities respectively. The zone of inhibition was compared with standard drug after 24 hours of incubation at 25oC for antibacterial activity and 48 hours at 30oC for antifungal activity. Results are tabulated in Table 2.
CONCLUSION: A simple and convenient method has been developed to synthesize novel 3 - (benzo [d] imidazole – 2 - yl amino) - 2- (3 - aryl- 1 – phenyl - 1H - pyrazole - 4 - yl) thiazolidin- 4- one (4a- f). Further, it reveals that the presence of pyrazole moiety attached to benzimidazole ring enhances the antibacterial activity.
TABLE 2: ANTIMICROBIAL ACTIVITY OF SYNTHESIZED COMPOUNDS
COMPOUND |
DOSE (µG/ML) |
ANTIBACTERIAL ACTIVITY | ANTIFUNGAL ACTIVITY | |||
ZONE OF INHIBITION (MM) | ZONE OF INHIBITION (MM) | |||||
S. AUREUS | P. VULGARIS | P. AERUGINOSA | A. NIGER | A. FLAVUS | ||
3a | 1000 | 08 | 10 | 16 | 20 | 18 |
3b | 1000 | 12 | 12 | 18 | 16 | 16 |
3c | 1000 | 10 | 14 | 10 | 18 | 20 |
3d | 1000 | 14 | 08 | 16 | 20 | 06 |
3e | 1000 | 06 | 16 | 14 | 18 | 14 |
3f | 1000 | 06 | 08 | 10 | 14 | 16 |
4a | 1000 | 14 | 11 | 12 | 20 | 12 |
4b | 1000 | 12 | 10 | 06 | 18 | 14 |
4c | 1000 | 06 | 14 | 06 | 14 | 10 |
4d | 1000 | 12 | 14 | 14 | 08 | 10 |
4e | 1000 | 10 | 16 | 06 | 12 | 18 |
4f | 1000 | 14 | 18 | 16 | 12 | 16 |
Control (DMF) | - | 0 | 0 | 0 | 0 | 0 |
Standard
(Gentamycin & Fluconazole) |
1000 | 16 | 22 | 20 | 22 | 18 |
** Zone of inhibition excluding well size 6mm
REFERENCES:
- Puratchikody A, Sivajyothi V, Jaswanth K and Nallu M: Synthesis, Reactivity and Biological Activity of Benzimidazoles. Indian J Heterocyclic Chem. 2002; 11:
- Pandey V. K and Raj N: Synthesis of α methylarylamido-ß-naphthyl-(1- Methylamino-2-methyl-benzimidazolyl)-ethers Curr, Sci. 1984; 53:256.
- Sengupta A. K, Gupta A. and Chandra U: Synthesis of 1-(4-carboxy 3-hydroxy phenyl aminomethyl) benzimidazole. Indian Drugs. 1985; 18:233.
- Wright J. B: Synthesis, Spectral Studies and Anti-inflammatory Activity of Some New 2-Aryloxybenzimidazoles Chem Rev. 1981; 48:
- Preston P.N: Synthesis of some substituted benzimidazole with potential antimicrobial activity. Chem Rev. 1974; 74:
- Zou R, Kawashima E, Freeman G.A, Koszalka G.W, Drach J.C and Townsend L.B: Design, Synthesis and Antiviral Evaluation of Some Polyhalogenated Indole C-nucleosides. Nucleosides Nucleotides and Nucleic acids. 2000; 19:125.
- Abdullah S, El-Assiery, Hosni G, Ahmed Fouda: Synthesis of some new annulatedpyrazolo-pyrido (or pyrano) pyrimidine, pyrazolopyridine and pyranopyrazole derivatives. Acta Pharma, 2004; 54:143.
- Lee K. Y, Kim J. M and J. N. Kim: Synthesis of 3-Benxyl-2-hydroxy-7, 8- dihydro-6H-quinolin-5-ones from Baylis-Hillman Adducts.Bull.Korean Chem. Soc.2005; 26:319.
- Hogale M. B, Uthale A. C and Nikam B. P: Synthesis and biological activity studies of some thiazolidinones and azetidinone. Indian J Chem., 1991; 30B:717.
- Asati K. C, Srivastava S. K and Srivastava D. S: Synthesis of 5-arylidene-2-aryl-3(benzotriazoloacetamidyl)-1, 3-thiazolidin-4-ones as analegesic and antimicrobial agents. Indian J Chem., 2006, 45B:526.
- Cesur N and Gursoy A: Synthesis and anticonvulsant activity of clubbed thiazolidinone-barbituric acid and thiazolidinone-triazole derivatives Arch Pharm., 1992; 325:
- Goudgaon N. M, Dhondiba V and Vijayalaxmi A: Synthesis and Antimicrobial Activity of N-1 Substituted Benzimidazoles. Indian J Heterocyclic Chem., 2004; 13:271.
- Jeelan Basha N, Upendar Reddy CH and Goudgaon N. M: Cyclization of 4-(2-Aminoanilino)-2-Benzylthiopyrimidine to Novel 1-(2-Benxylthiopyrimidin-4-yl)-2-Subtituted Benzimidazoles. Heterocyclic Communications, 2008; 14:469.
- Goudgaon N. M and Jeelan Basha N: Synthesis and Antimicrobial Activity of 2-(-Benzylthiopyrimidn-4-yl) substituted Benzimidazoles. Indian J Heterocyclic Chem., 2009; 18:353.
- Goudgaon N. M, Jeelan Basha N and Sharanabasappa B. Patil: Synthesis of 2-Benzylthiopyrimidinyl Pyrazole Analogs and Their Antimicrobial Activities. Indian J Heterocyclic Chem., 2009; 18:349.
- Jagath Reddy G, Latha D and Srinivas Rao K: Synthesis of some new Ethyl-4-(1, 3- Diarylpyrazol-4-yl)-6-Methyl-2-thioxo-1, 3, 4-trihydropyrimidine-5-carboxylates Heterocyclic Communications, 2004; 10:331.
Article Information
6
50-56
464 kB
1079
English
IJPSR
Sharanabasappa B. Patil and Naganna M. Goudgaon*
Department of Studies and Research in Chemistry, Gulbarga University, Gulbarga (Karnataka), India
ngraghu@rediffmail.com
05 March, 2010
20 April, 2010
20 May, 2010
http://dx.doi.org/10.13040/IJPSR.0975-8232.1(6).50-56
01 June, 2010