TARGETED DELIVERY OF CATALASE TO MACROPHAGES BY USING SURFACE MODIFICATION OF BIODEGRADABLE NANOPARTICLEAbstract
Reactive oxygen species (ROS) produced by activated macrophages play a major role in causing liver disorders. Small antioxidants and scavengers are protective only at very high concentrations and they poorly detoxify toxic ROS. Catalase (CAT) is an antioxidant enzyme that scavenges ROS and has great potential for treating liver disorders. In the present research work, gelatin nanoparticles bearing CAT were prepared using a two-step desolvation technique coupled with mannose and targeted in to liver to treat liver disorders associated with excess of ROS. It was characterized for shape, particle size, zeta potential, and percentage drug release and entrapment and confirmed by infrared spectroscopic studies. Experimental studies demonstrated that mannose Coupling enhanced their uptake into macrophages due to presence of mannose receptors in macrophages which increases their ability to scavenge ROS, produced by the activated macrophages.
Ajay Saraf *, Amit Dasani and Ashok Soni
Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Central University, Sagar, Madhya Pradesh, India
05 February, 2013
10 April, 2013
20 May, 2013
01 June, 2013