THE ANTIOXIDANT EFFECTS OF IPB-3S STRAIN ORYZA SATIVA BRAN EXTRACT TOWARDS MALONDIALDEHYDE AND GLUTATHIONE PEROXIDASE LEVELS IN RAT BRAIN INDUCED BY CARBON TETRACHLORIDEAbstract
IPB-3S Strain Oryza sativa bran, a high-antioxidant food, was expected to protect the brain as a susceptible organ from oxidative damage. This study was conducted to analyze the effect of rice bran extract on malondialdehyde (MDA) and glutathione peroxidase (GSH) levels in the brain induced by carbon tetrachloride (CCl4). A total of 24 Sprague-Dawley male rats was divided into 6 groups, including normal control (C1); negative control (C2); 150 mg/kg rice bran extract (I1); 150 mg/kg rice bran extract + CCl4 (I2); 300 mg/kg rice bran extract (I3); and 300 mg/kg rice bran extract + CCl4 (I4). The MDA levels were measured by Wills method, while GSH levels were measured by the Ellman method. The administration of low-dose (150 mg/kg) and high-dose (300 mg/kg) rice bran extracts significantly lowered MDA levels compared to control groups, both in the groups with and without the induction of CCl4 (p <0.05). Moreover, the administration of low-dose (150 mg/kg) also significantly increased GSH level (p <0.05), contrary to the groups administered by high-dose (300 mg/kg) rice bran extracts, which relatively reduced GSH level. In conclusion, rice bran extract has been proven to increase antioxidants in rat brain tissue by reducing MDA levels and increasing GSH levels.