THE EFFECT ON CELL VIABILITY AND REDUCTION OF CORTISOL ACTIVITY BY AQUEOUS EXTRACTS OF GREEN COFFEE BEAN

A recent surge of lifestyle disorders such as obesity, Type II Diabetes Mellitus and cardiovascular diseases, has led to a search of dietary changes that could be adopted into the daily lifestyle to prevent these lifestyle disorders. A nutraceutical is an upcoming option that has garnered a lot of attention and has warranted a lot of research. Green coffee beans are unroasted coffee beans which have high levels of antioxidants and caffeine thus making it a potential nutraceutical against Type II Diabetes Mellitus and obesity. In this study, water-soluble brews of caffeinated and decaffeinated green coffee beans were used to evaluate the ability of these extracts to alter the viability of muscle cells of Sus scrofadomesticus and also their effects on cortisol-induced (stress) hepatocytes by ex-vivo studies. The green coffee bean extracts were found to have low cytotoxicity. These extracts also highly controlled the release of glucose and regulated the gluconeogenic pathway by regulating glucose synthesis induced by cortisol in Sus scrofadomesticus hepatocytes when compared with the standards.