UV-SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF LORNOXICAM AND EPERISONE HYDROCHLORIDE IN BULK DRUG, FORMULATIONS AND AQUEOUS DISSOLUTION SAMPLESAbstract
Lornoxicam has been found to be not interacted by eperisone hydrochloride at 377 nm. Therefore, it is estimated directly as a single component in the mixture, whereas eperisone hydrochloride is throughout interfered with by lornoxicam in UV region. So, it was estimated in the mixture using a two-wavelength difference method. Lornoxicam was found to have same absorbance at 262 nm and 283 nm but not eperisone hydrochloride. So, a difference in absorbance of the mixture at 262 nm and 283 nm was directly proportional to the concentration of eperisone hydrochloride. The calibration curves were validated according to ICH guidelines and were linear over the concentration range tested (10-20 µg/ml for lonoxicam and 3-24 µg/ml for eperisone hydrochloride). The curves were found to be precise and accurate as shown by acceptable limits of %RSD (0.37%-0.66%) and percent recoveries (98.73% ± 1.02%-102.44% ± 0.90%) respectively for both the drugs in mixture as well as in pure solutions.
N. Prasad *, P. Singh and P. Kumar
SGT College of Pharmacy, SGT University, Gurugram-Badli Road, Budhera, Gurugram, Haryana, India.
29 May 2019
12 October 2019
04 December 2019
01 April 2020