VALIDATED HPTLC METHOD FOR ESTIMATION OF CURCUMIN CONTENT IN DIETARY SUPPLEMENT FORMULATION
AbstractThe simple, accurate and precise HPTLC method was developed for quantification of curcumin content in dietary supplement formulation. In this method, individual curcuminoids (curcumin, demethoxy curcumin and bisdemethoxy curcumin) with piperine were resolved using mobile phase n-hexane: ethyl acetate: methanol: formic acid (8: 2: 1: 2-3 drops v/v) on a plate precoated with silica gel 60 F254 and quantified densitometrically in absorbance mode at 421 nm. The Rf value of curcumin was found to be 0.29. Linearity for curcumin was established between concentration range of 100-180 ng/spot with correlation coefficient of 0.999. The method was further validated as per ICH guidelines. The LOD and LOQ values for curcumin were found to be 27.3 ng and 82.7 ng respectively. The results of percent recovery and repeatability studies with standard deviation (≤2%), concluded that the developed method was accurate and precise and can be used for routine analysis of curcumin in dietary supplement formulations
Article Information
35
3796-3800
599KB
1525
English
Ijpsr
V.A. Kekre* and S.G. Walode
Department of Pharmaceutical Chemistry, Sinhgad Institute of Pharmaceutical Sciences, Off Mumbai - Pune Express way Kusgaon (B.K), Lonavala-410401, Maharashtra, India
vaibhavkekre@gmail.com
01 June, 2012
10 July, 2012
19 September, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(10).3796-00
01 October, 2012
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