VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR IN-VITRO DISSOLUTION STUDIES IN PHOSPHATE BUFFER pH 7.4Abstract
A simple, accurate, and economical, least time-consuming method has been developed for Losartan potassium (LP) by using UV spectrophotometer. This method was developed using phosphate buffer solution pH 7.4 (PBS) for quantifying the amount of LP released from the colon-specific formulations. The wavelength maximum (λmax) of LP in phosphate buffer pH 7.4 was found to be 228.5 nm. The developed UV spectroscopic method exhibited linearity in the range of 2-10 μg/mL with a correlation coefficient (R2) value of 0.999 and was validated with respect to linearity, specificity, accuracy (recovery), and precision. These parameters were determined according to International Conference on Harmonization (ICH) guidelines. Results of the analysis were validated statistically and by recovery studies, which proved the suitability of the developed method for the routine estimation of LP in colon-specific control release formulation. Hence, the reported method for the estimation of LP from the colon-specific formulations was simple, accurate, and least time-consuming.
S. Chaturvedi *, S. Ayaz and K. Shah
Institute of Pharmaceutical Research, GLA University, Mathura, Uttar Pradesh, India.
03 April 2020
22 July 2020
16 August 2020
01 April 2021