VALIDATION AND DEVELOPMENT OF TLC-DENSITOMETRY METHOD FOR STANDARDIZATION OF SOURSOP LEAF EXTRACT (ANNONA MURICATA LINN.) WITH QUERCETIN

Standardization is a process involving various chemical analysis methods based on pharmacological data involving general physical and microbiological analyzes aimed at the safety of a natural extract. To obtain the correct result, the standardization step is preceded by method validation. Quercetin can be obtained from the leaves of soursop (Annona muricata Linn.), which use for treatment fever, diarrhea, anti-convulsive, antifungal, antimicrobial, gout, itching, and flu. Determination of quercetin in soursop leaf extract using TLC-Densitometry must be validated by testing selectivity, limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy, and range. Densitometry scanning of the plates silica gel 60 F₂₅₄ directly at 265 nm was used for analysis of quercetin. The result of selectivity test was 1.53 (>1.5) with mobile phase toluene: ethyl acetate: formic acid (4: 3: 0.4; v/v/v), limit of detection (LOD = 0.018 µg), limit of quantification (LOQ = 0.060 µg), linearity y = 9387.3x – 767.39 (r = 0.9996 and Vxo = 1.9%), Coefficient of Variation (C.V.) of precision 1.906525% and the percent recovery of 96.6% ± 8.26%. The validated and development method was applied for standardization of quercetin in soursop leaf extract and resulted in the quercetin assay in soursop leaf extract was 1.288% ± 9.996% (w/w).