VARIATION IN β – SITOSTEROL CONTENT FROM CASSIA FISTULA L. FRUIT PULP COLLECTED FROM DIFFERENT GEOGRAPHICAL REGIONSAbstract
Cassia fistula L. (Fabaceae) fruits are reported to possess a wide range of therapeutic properties. In the present work, an HPTLC method has been developed and validated for estimation of β-sitosterol content in C. fistula fruit collected from different geographical regions in and outside India and its polyherbal formulation. Chromatographic separation was achieved on HPTLC plates pre-coated with silica gel 60 F254 using toluene: ethyl acetate: methanol (7:1:0.5, v/v/v) as a mobile phase. A compact spot of β-sitosterol at Rf value of 0.46 ± 0.02 was observed in all the samples of C. fistula by derivatizing the HPTLC plate with 10 % methanolic sulphuric acid reagent followed by its densitometric scanning using CAMAG TLC scanner 4 at 366 nm. The method was validated as per ICH guidelines. Finding of the present work can be used as a routine quality control tool for the assessment of C. fistula fruits in terms of its β-sitosterol content.
S. Shailajan*, M. Yeragi, A. Matani and D. Gurjar
Herbal Research Laboratory, Ramnarain Ruia College, Matunga (E), Mumbai 400019, Maharashtra, India
26 June, 2013
23 July, 2013
25 October, 2013
01 November, 2013