VASORELAXATION EFFECT PROMOTED BY THE CALCIUM CHANNEL BLOCKER VERAPAMIL COORDINATED TO RUTHENIUM (II): CHEMICAL AND BIOLOGICAL PROPERTIES
AbstractThis paper presents the synthesis and physical-chemical characterization of a new terpyridine (tpy) ruthenium complex coordinated to the L-type calcium blocker channels verapamil (vera) obtained from commercial pill. Elemental analysis (%C N H), infrared (IR) and ultraviolet-visible (UV-vis.) spectroscopy, mass spectrometry (MS), and 13CNMR (nuclear magnetic resonance), and HPLC (high performance liquid chromatography) confirmed the pure and structure of the ligand verapamil and of the ruthenium complex structurally designed as [RuIICl2(tpy)(vera)] Cyclic voltammetry aided investigation of the electrochemical behavior of [RuIICl2(tpy)(vera)] based in the metal ion redox potential. Verapamil ligand and [RuIICl2(tpy)(vera)] induced vascular relaxation in endothelium-intact and endothelium-denuded aortic rings. Verapamil drug and [RuIICl2(tpy)(vera)] complex coupled to verapamil elicited similar pharmacological results. The calcium ion chelating effects (Kd) promoted for verapamil ligand and [RuIICl2(tpy)(vera)] complex were found based in the fluorescence technic. The smooth muscle cell images studied to [RuIICl2(tpy)(vera)] complex acting as a luminescent agent important as biological probe
Article Information
10
3733-43
719
1326
English
Ijpsr
O. A. Nascimento, E. F. Boffo , B. R. Silva, J. C. Biazzotto, L. M. Bendhack, R. S. da Silva and R. G. de Lima
Departamento de Química , Universidade Federal da Bahia, Avenida Barão de Geremoabo number 147 , Ondina, Salvador, BA, Brazil
renatagalvao@pontal.ufu.br
08 February, 2015
19 March, 2015
07 June, 2015
10.13040/IJPSR.0975-8232.6(9).3733-43
01 September, 2015
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