CHEMICAL PROFILING, ADME PREDICTION OF BOERHAVIA DIFFUSA LINN. AND CRATEVA NURVALA BUCH. HAM IN THE MANAGEMENT OF UROLITHIASISAbstract
Background: This study was carried out to investigate qualitative and quantitative phytochemical analysis and free radical scavenging activity of Boerhavia diffusa (root) and Crateva nurvala (bark) along with certain computational ADME (Absorption, Distribution, Metabolism, Excretion) parameters of compounds analyzed from Boerhavia diffusa (root) and Crateva nurvala (bark). Methods: The dried powder of plants were extracted with increasing polarity of solvent namely ethyl acetate, acetone, ethanol and distilled water by a three-step sequential extraction procedure. The chemical compositions of extracts were investigated using UV-Vis, FTIR and PerkinElmer Gas Chromatography-Mass Spectrometry. Ethanolic extract of Boerhavia diffusa and Crateva nurvala was evaluated in vitro for its ability to inhibit the major enzyme activities of GOX and LDH through spectrophotometrically and mode of inhibition were evaluated using Lineweaver-Burk plots while ADME parameters were derived from ADMET server admet SAR. Results: Among all extracts, ethanolic extracts showed the highest phenol, flavonoid, proanthocyanidins, and coumarin content. High DPPH free radical quenching activity was observed in ethanolic, ethyl acetate and acetone extracts of the plant which was significantly comparable with ascorbic acid. Also, plant extracts, analyzed by UV-FTIR and GC-MS which showed promising Glycolate oxidase and Lactate dehydrogenase inhibition and found to contain phenolic, glycosides and flavonoids compounds. Conclusion: The FTIR and GC-MS spectrum profile of the medicinally important plant extract having various bioactive compounds and interestingly from the absorption, distribution, metabolism, and excretion (ADME)-Toxi-city analysis the compound have enhanced pharmacological properties and can be used as an herbal alternative for the synthesis of antiurolithiac agents.
S. R. Gophane * and C. N. Khobragade
School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, India.
14 June 2018
14 August 2018
31 August 2018
01 February, 2019