COMPARATIVE ACUTE AND SUB-ACUTE TOXICITY STUDY OF HYDRO-ALCOHOLIC EXTRACTS OF CENTELLA ASIATICA AND EVOLVULUS ALSINOIDES IN SWISS ALBINO MICEAbstract
In this study, the in-vivo toxicity of Centella asiatica & Evolvulus alsinoides were evaluated by acute and sub-acute toxicity assays according to the guidelines of OECD 423 & 407 respectively. For LD50 evaluation, a single dose of hydro-alcoholic extracts of both plants was orally administered to Swiss albino mice at doses of 200, 400, 800, 1600 and 2000 mg/kg. Then the animals were observed for 72 h. For acute toxicity evaluation, a single dose of hydro-alcoholic extracts of both plants was orally administered to mice at doses of 300, 600, 1200 and 2000 mg/kg. Then, the animals were observed for 14 days. In the sub-acute study, the extracts were orally administered to mice for 28 days at doses of 300, 600, 1200, and 2000 mg/kg. To assess the toxicological effects, animals were closely observed on general behavior, clinical signs of toxicity, body weight, food, and water intake. At the end of the study, it was performed biochemical and hematological evaluations, as well as histopathological analysis from the following organs: brain, heart, liver, and kidney.
M. K. Yadav *, S. K. Singh, M. Singh, S. S. Mishra, A. K. Singh, J. S. Tripathi and Y. B. Tripathi
Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
07 February 2019
28 May 2019
14 June 2019
01 October 2019