EVALUATION OF IRON OXIDE NANOPARTICLES (NPs) ON AGING AND AGE RELATED METABOLISM AND PHYSIOLOGICAL CHANGES IN C. ELEGANSAbstract
In recent decades, advances in nanoscience have resulted the rapid development of many novel technological applications for nanoparticles (NPs) in various in Biomedical Sciences. Iron is essential for eukaryotic biochemistry and is an important element in several metabolic processes such as, carrier for oxygen transportation, cell respiration, synthesis of genetic material and protein synthesis. The nematodes Caenorhabditis elegans (C.elegans) represent an excellent organism to investigate the phenotypic effect of different type of nanomaterials on the molecular, cells and entire organism level. C.elegans is widely used in studies of homeostasis, neurolagenerative and metal toxicity. The size of iron oxide and other nanoparticles plays a major role in target cell uptake and elimination from the body. The excess iron damage nucleotides (DNA/RNA) and Protein due to catalyzes the generation of Reactive Oxygen Species (ROS) , while cellular iron deficiency causes cell cycle arrest and cellular death of an organism. Systematic storage and trafficking is required to regulate supply of iron while preventing it from catalyzing unwanted reactions. We have used 36 nm size iron oxide nanoparticles, prepared by Ayurvedic method and investigated the effect of manipulating iron oxide nanoparticles on aging and age related phenotype. Iron oxide nanoparticles enhance the toxicity in C.elegans. In this present article, we report phenotypically study of iron oxide nanomaterial on age-related elevation, average life span, bending and spontaneous movement. The damaging reactive oxygen species which causes decreases the average life span of C.elegans.
S. Kumar, R. K. Singh, A. K. Aman*, J. Kumar and M. Kar
Aryabhatta Centre for Nanoscience and Nanotechnology, Aryabhatta Knowledge University, Patna, Bihar, India.
07 February, 2017
26 July, 2017
11 August, 2017
01 September, 2017