IN-VITRO ANTIOXIDANT ACTIVITY OF THE SUCCESSIVE EXTRACTS OF RICINUS COMMUNIS STEMSAbstract
There is increasing evidence to support the involvement of free radical reactions in several human diseases. n recent years, it has become increasingly apparent that in man, free radicals play a role in a variety of normal regulatory systems, the de-regulation of which may play an important role in inflammation. Active oxygen species and other free radicals have long been known to be mutagenic. Further, these agents have more recently emerged as mediators of the other phenotypic and genotypic changes that lead from mutations to neoplasia. Therefore, free radicals may contribute widely to cancer development in humans. The antioxidant activities of the plant extract and pure compounds were assessed on the basis of radical scavenging effect of the stable DPPH free radical. Generally plants containing flavonoids having strong antioxidant properties. The six extracts Ricinus communis stem and two standards tested for antioxidant activity using DPPH method, the benzene and 50% methanol successive extracts showed the maximum antioxidant activity with IC50 values of 36.19 ± 2.332 mg/ml and 34.40 ± 5.98 mg/ml, respectively. The methanol and chloroform extract also showed antioxidant activity with IC50 values of 64.18 ± 3.20 and 66.17 ± 6.30 mg/ml. The distilled water crude extracts showed IC50 values of 106.14 ± 4.33 mg/ml, respectively.
Ramesh Kumar Singh *, M K Gupta, Deepti Katiyar, Anshul Srivastava and Parul Singh
Department of Pharmacy, Rameshwaram Institute of Technology & Management, Lucknow (UP) India
15 April, 2010
16 June, 2010
03 August, 2010
15 August, 2010