ASSESSMENT OF ANTIOXIDANT, ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE POTENTIAL OF POLYHERBAL FORMULATION

Diabetes mellitus is a group of disorders that speed up the metabolism of protein, fat, and carbohydrates. It has been forecasting rises in the incidence and prevalence of diabetes mellitus in recent years. Different plant parts have historically been used to treat diabetes mellitus. The Plant Gmelina arborea (lamiaceae) treats insecticidal, antidiuretic, anti-diabetic, anti-analgesic, and immunomodulatory activity. Basella rubra (Baellaeceae) is used as an Androgenic, diuretic, emollient, demulcent and anticonvulsant. Sphaeranthus indicus (Asteraceae) treats ananxiolytic, neuroleptic, antioxidant, anti-inflammatory, bronchodilatory, antihyperglycemic and hepatoprotective. The plants were processed, powdered and extracted with hydroalcoholic solvent. Maceration was processed for 72hrs, 48 hrs and 24 hrs. Solvents were filtered, distilled, and dried in a vacuum desiccator. Polyherbal formulations such as BSG, SBG, BGS were made by taking the required proportion of each powdered drugs. The in-vitro anti-oxidant and anti-diabetic potential of plant were determined by DPPH radical scavenging activity, Alpha-amylase and Alpha-glucosidase inhibition assay. The activity reported that hydroalcoholic extract of Gmelina arborea, Basella rubra, and Sphaeranthus indicus exhibits potent activity indose–dependent manner. The Polyherbal formulation is used to treat diabetics with fewer adverse effects.