DEVELOPMENT OF VALIDATED HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF FERULIC ACID AND ELLAGIC ACID
AbstractA simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and completely validated for the estimation of ellagic acid and ferulic acid in herbal formulation. Quantification was carried out with pre-coated silica gel aluminium plate G60F254 (10x10cm) as stationary phase, mobile phase consisting of Ethyl acetate: toluene: formic acid: methanol (3.5: 4:0.8:0.2 v/ v/ v) and scanned in Absorbance Reflectance mode at 275 nm using Camag TLC scanner 3 with WinCAT software. The Rf value of 0.31 for ellagic acid and 0.54 for ferulic acid was obtained. The proposed method has permitted the quantification of ellagic acid and ferulic acid over the linearity range of 100 to 1000 ng/spot, its percentage recovery was calculated along with intraday and inter day precision. The limit of quantification and the limit of detection were found to be 38.96ng/ spot and 12.85ng/ spot, respectively for ellagic acid and 42.28ng/spot and 27.66ng/spot, respectively for ferulic acid. The suggested method can be successfully used to estimate the drug content of many commercial formulations on a single plate.
Article Information
46
5994-5999
621 KB
448
English
IJPSR
V. M. Jadhav *, V. J. Kadam, S. S. Pawar and A. R. Khobarekar
Department of Quality Assurance, Bharati Vidyapeeth’s College of Pharmacy, University of Mumbai, Navi Mumbai, Maharashtra, India.
varsha.jadhav@bvcop.in
03 May 2023
24 June 2023
05 July 2023
10.13040/IJPSR.0975-8232.14(12).5994-99
01 December 2023
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