HEPATOPROTECTIVE ACTIVITY OF SINIGRIN AND CARVACROL AGAINST PARACETAMOL INDUCED LIVER INJURY IN WISTAR RATS

Background: Sinigrin (allyl-glucosinolate or 2-propenyl-glucosinolate), an aliphatic glucosinolate, is naturally present in Brassicaceae plants, while carvacrol (2-methyl-5-isopropylphenol) is a monoterpene phenolic component of the essential oil generated by many aromatic plants. This study is aimed to determine the hepatoprotective potential of these phytoconstituents on Paracetamol-induced liver damage in Wistar rats. Methods: Seven groups of six Wistar rats, each weighing between 180 and 230 g, were selected. The animals’ weight range was evenly divided throughout the groups. The control group, group I, got normal saline; the PCM group, group II, received paracetamol (3 g/kg, p.o.); groups III and IV, carvacrol (25, 50 mg/kg/p.o.); and the groups V and VI, sinigrin (25, 50 mg/kg/p.o.) once daily for 14 days. Silymarin was given daily to group VII- Standard at a dose of 25 mg/kg p.o. Result: Serum samples were used to determine levels of marker enzymes such as aspartate transaminases (AST), alanine transaminases, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and glutamyl transpeptidase (GGT). The proinflammatory cytokines interleukin-1beta (IL-1b) and interleukin-6 (IL-6) as well as nitrite levels and catalase activity all decreased in carvacrol and sinigrin-treated rats in a dose-dependent manner. Conclusion: The present study will provide scientific data and justify their traditional uses to treat various liver problems. This study confirmed that carvacrol and sinigrin have hepatoprotective potential against Drug Induced Liver Injury (DLI).