CHEMICAL CHARACTERISATION, EVALUATION OF ANTIOXIDANT ACTIVITY AND ACUTE TOXICITY OF POLAR EXTRACTS OF TABERNAEMONTANA CRASSA BENTH (FAMILY APOCYNACEAE)
AbstractTabernaemontana crassa Benth, synonym Tabernaemontana jollyana Pietre ex Stapf, is a Central African pharmacopoeia plant whose stem bark is widely used in traditional medicine. The aim of this study was to evaluate the acute toxicity of aqueous extracts of this species. Preliminary phytochemical tests carried out on the different crude extracts of T. crassa revealed the following chemical groups: alkaloids, quinones, triterpenes and sterols. Quantitative analysis showed that the hydro-methanolic and methanolic extracts of T. crassa had a high content of total polyphenols and flavonoids (247,33 ± 2,05 mg Eq GA/g Ms and 1543,93 ± 10,15mg Eq Rut/g Ms, respectively). Evaluation of the free radical scavenging capacity of T. crassa extracts (methanolic, hydro-methanolic and aqueous) using the DPPH free radical scavenging method on 96-well microplates showed moderate IC50 values (0,2714 ± 0,0020, 0,2480 ± 0,0006 and 0,3129 ± 0,0005 mg/mL), but still low compared to the reference molecule, ascorbic acid (0,064 ± 0,000 mg/mL). The acute toxicity study of the aqueous extract showed no clinical signs of toxicity at single doses of 2000 and 5000 mg/mL body weight in mice.
Article Information
11
1099-1106
591 KB
423
English
IJPSR
Ngaïssona Paul *, Namkona Frédéric Armel, Koane Jean Noël, Etou-Ossibi Arnaud Wilfried, Nkounkou Loumpangou Célestine, Syssa-Magalé Jean-Laurent and Ouamba Jean-Maurille
Laboratory of Architecture, Analysis and Reactivity of Natural Substances, Faculty of Sciences, University of Bangui, BP.908 Bangui.
pngais@yahoo.fr
19 September 2023
08 December 2023
21 March 2024
10.13040/IJPSR.0975-8232.15(4).1099-06
01 April 2024
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