ANTIOXIDANT AND CHOLINESTERASE INHIBITORY ACTIVITY OF GREWIA HIRSUTA VAHL. LEAVES
AbstractObjectives: Grewia hirsuta Vahl. is a shrub explored for medicinal applications. It has anti ageing, boosting immunity, prevents loss and grey hair etc. Ethnopharmacological data suggests that the plant is extensively used as a nerve tonic and to enhance the cognitive properties. The basis of these medicinal uses seems to be antioxidant and anti cholinesterase activity. In order to establish the scientific basis between medicinal uses and its mechanism of action, antioxidant and anti cholinesterase activity investigations were carried out. Methods: Evaluation of free radical scavenging and anti cholinesterase activity was carried out using DPPH (1, 1- diphenyl-2-picryl hydrazyl), hydroxyl radical scavenging and ferric reducing method using ascorbic acid as a standard. Cholinesterase inhibitory potentials were measured by Ellman’s method against Rivastigmine. Results: The inhibitory concentration of hydro ethanolic extract of Grewia hirsuta (HEEGH) (IC50 27.25) in DPPH. In reducing power assay (IC50 65.32) and Hydrogen peroxide scavenging activity (IC50 98.28). The inhibitor concentration of acetylcholinesterase by standard (Rivastigmine) was (IC50 14.40μg/ml), and the extract was (IC50 133.4μg/ml), whereas, butyrylcholinesterase (IC50 96.46μg/ml). Conclusion: HEEGH possesses the significant concentration-dependent inhibitory action as an antioxidant and anti acetyl cholinesterase and butyrylcholinesterase activity. This activity might be due to the presence of phenols, flavonoids, tannins and specific amino acids in significant amount.
Article Information
37
2495-2499
556 KB
49
English
IJPSR
V. Chikkamath *, V. H. Kulkarni, P. V. Habbu, A. N. Nagappa and G. M. Sindhu
Department of Pharmacology, S. C. S. College of Pharmacy, Harapanahalli, Karnataka, India.
vinuth6288@gmail.com
09 March 2024
09 April 2024
24 April 2024
10.13040/IJPSR.0975-8232.15(8).2495-99
01 August 2024