GLUCOREGULATORY EFFECT OF METHANOLIC EXTRACT OF PICRALIMA NITIDA SEEDS IN RATS MADE DIABETIC BY ALLOXANE
AbstractDiabetes is a chronic disease characterized by fasting blood glucose levels above 1.26 g/L. The aim of the present study was to evaluate the effect of the methanolic extract of Picralima nitida seeds on insulin and blood glucose levels in diabetic rats. Diabetes was induced in normoglycemic rats by injection of 1 mL alloxane at a dose of 125 mg/kg bw for three consecutive days. Blood samples were taken every other day to determine insulin and blood glucose levels. Diabetic rats were then treated for 14 days with 10, 20 and 50 mg/kg bw of P. nitida seed extract and 5 mg/kg bw of Glibenclamide®. At the end of treatment, the rats’ blood was collected again for determination of insulin and blood glucose P. nitida levels. The results obtained show that initial insulinemia, which was 16 ± 0.12 μU/mL, fell in diabetic rats (7 ± 0.004 μU/mL) before significantly increasing to 15 ± 0.3 μU/mL and 17 μU/mL ± 0.02 after treatment with P. nitida extract and Glibenclamide®. In addition, initial blood glucose levels, estimated at 0.76 ± 0.022 g/L, increased in rats following alloxane injection and then significantly fell to 0.65 ± 0.06 g/L and 0.55 ± 0.03 g/L in animals treated with extract and Glibenclamide®. These results indicate that the methanolic extract of Picralima nitida seeds, like Glibenclamide®, is capable of regulating blood insulin and glucose levels in diabetic rats.
Article Information
45
2556-2564
631 KB
47
English
IJPSR
Tiekpa Wawa Justine *, Kouassi Konan Armand Marcelin, Kouamé Yves, Touré Abdoulaye, Soro Yadé Réné, Sea Tehi Bernard and Bahi Calixte
Laboratory of Biotechnology and Valorisation of Agroressources and Natural Substances, Training and Research Unit of Biological Sciences, Peleforo Gon Coulibaly University of Korhogo, PO BOX 1328 Korhogo, Côte d’Ivoire.
tiekpajustine@yahoo.fr
12 February 2024
05 June 2024
19 June 2024
10.13040/IJPSR.0975-8232.15(8).2556-64
01 August 2024