COMPARING DIFFERENT DOSES OF PENTYLENETETRAZOLE INDUCED EXPERIMENTAL MODEL OF SEIZURE IN MICE

Background: Epilepsy, characterized by repetitive seizures, is studied using pentylenetetrazole (PTZ) in animals. PTZ, a GABA-A inhibitor, is used in various doses in mice to evaluate drug efficacy against seizures. Method: In a PTZ-induced mouse model, different PTZ doses were evaluated: 80 mg/kg i.p., 75 mg/kg i.p., and 80 mg/kg s.c. Seizure behaviors were analyzed, including mortality rates, GTCS frequency and latency, myoclonic jerks, GTCS duration, and post-injection interictal phase duration. Results: Frequency of GTCS was found to be highest, latency to GTCS and myoclonic jerks was least and duration of GTCS was more in PTZ 80 mg/kg, i.p., group, while the PTZ 80 mg/kg; s.c. was found to have longest inter-ictal phase. These are important criterion for the evaluation of any investigational drug for antiepileptic effect. The mortality rate was found to be the same in PTZ 75 mg/kg; i.p. and 80 mg/kg; s.c. (12.5%) groups, while in PTZ 80 mg/kg; i.p. it was found to be highest (25%). The PTZ 80 mg/kg, i.p., for comparison of seizure parameters was found to be the most appropriate dose. Comparison with Existing Methods: This research was conducted to gain a deeper comprehension of acute seizures induced by PTZ in mice. Three doses of PTZ were compared on the basis of different seizure scoring parameters and mortality to better understand and visualize seizure episodes at various dosages.