BACTERIAL PROFILE AND ANTIBIOGRAM OF BODY FLUIDS ISOLATED FROM VARIOUS IPDS PATIENTS

Introduction: Pathogenic infections place a significant burden on the healthcare system since they are linked to high rates of morbidity and mortality. Successful therapy of these illnesses depends on an early diagnosis and a good antimicrobial resistance testing. Therefore, the goal of the current investigation was to assess aerobic bacteriological profile and antibiogram from a variety of sterile fluids. Material and Method: This observational study was carried out in the Department of Microbiology, Adesh Institute of Medical Science & Research Bathinda, Punjab from 20 September 2023 to 15 February 2024 obtaining the approval from Ethics Committee of Biomedical & Health Research, Adesh University. All the Body fluid samples were received in Bacteriology Laboratory from various departments. All body fluid samples are processed by Bact alert system. Positive samples were further subculture on blood agar and MacConkey agar. Gram Staining was done to identify gram-positive bacteria and gram-negative Bactria, further antimicrobial susceptibility was done by Vitek 2 compact system as per CLSI guidelines 2023. Results: A total of 288 body fluid samples were received, from which Pus & Swab 102 (38.05%) were highest in number. Among the 157 isolates, the most predominant organism was Escherichia Coli 37 (23.56%) followed by Staphylococcus aureus 29 (18.47%), Klebsiella pneumoniae 25 (15.9%). The most sensitive drugs for gram-positive isolates were Daptomycin, Teicoplanin, Vancomycin, Doxycycline, Minocycline and for gram negative isolates were Amikacin, Minocycline, Tigecycline, Fosfomycin. Conclusion: The study concluded that multidrug-resistant bacteria are mostly responsible for infections of sterile body fluids and their antibiotic resistance varies greatly across various geographic areas and healthcare settings. Therefore, empirical treatment and antimicrobial testing crucial for effective treatment.