MICROBIOLOGICAL OBSERVATION CONCERNING THE STABILITY PROFILE OF TRIPHALA CHURNA WITH REFERENCE TO BASELINE STUDY

Introduction: Triphala Churna a polyherbal Ayurvedic formulation – with significant therapeutic potential is used to treat a variety of oral ailments. To ensure product’s quality and effectiveness, it is essential to evaluate its microbiological stability. Materials and Methods: Triphala Churna was prepared using standard procedures and stored in various containers for ten months under different real-world storage conditions, without specific control over temperature or humidity. Microbial contamination was assessed periodically through Gram staining for bacteria, wet mount preparation for fungal visualization, and culture methods for isolating and identifying potential microbes. Discussion: No bacterial or fungal growth was observed in any of the samples throughout the ten-month study period, even under conditions that could potentially support microbial growth. The preparation, storage, and inherent qualities of the individual ingredients in the formulation may have contributed to the observed findings. Conclusion: This preliminary study indicates good microbial stability of Triphala Churna under real-world storage conditions. However, further research using a wider range of microbial detection methods is needed for a more comprehensive evaluation.