COMPARATIVE IN-VITRO STUDY OF ANTIMICROBIAL, ANTIFUNGAL OF SILVER NANOPARTICLES SYNTHESIZED FROM AQUEOUS AND ETHANOLIC LEAF EXTRACT OF GMELINA PHILIPPENSIS CHAM.

The development of a dependable green chemistry approach for the biogenic production of nanomaterials is an essential component of contemporary nanotechnology research.  The biosynthesis of metal nanoparticles is a widely acknowledged method that employs a bottom-up approach with reduction as the primary process. When compared to chemical approaches, the nanoparticles created by this method are safer, more cost-effective and less harmful to the environment. Metallic nanoparticles with bactericidal and inhibitory properties include silver nanoparticles. Pathogenic bacteria have become more resistant to antimicrobial medicines in recent years, which presents a significant challenge to the healthcare system. Additionally, the fast development of antibiotic resistance during this time has made scientists and researchers reconsider the therapeutic potential of silver and its nanoparticulate systems as possible antibacterial and antifungal agents. In the present investigation, we have comparatively studied the antimicrobial and antifungal activity of 1mM, 0.1 M solution of silver nitrate, silver nanoparticles synthesized from aqueous and ethanolic leaf extract and Cd- doped silver nanoparticles and antioxidant activity of aqueous and ethanolic extract of Gmelina philippensis Cham. For the antimicrobial activity we have used two gram-negative and two gram-positive pathogens. For antifungal activity, the fungus Candida albicans was used and DPPH method was used to examine the antioxidant activity.