OPTIMIZING AEGLE MARMELOS TISSUE CULTURE: STERILIZATION, HORMONAL COMBINATIONS, AND PERMEABILIZATION STRATEGIES FOR ENHANCED SECONDARY METABOLITE PRODUCTION

This study focuses on optimizing the tissue culture of Aegle marmelos, a medicinal plant with diverse pharmacological properties. The research fine-tuned sterilization methods, identifying a 2% sodium hypochlorite solution as the most effective. Callus induction experiments revealed the optimal hormonal combination for Aegle marmelos as MS medium + Auxin-2,4-D (0.5 mg/l) + Cytokinin-BAP (1.5 mg/l). Suspension cell cultures were then developed as a foundation for further experiments. The study also explored permeabilizing agents (Triton X-100 and Tween-80) in Aegle marmelos cell cultures, finding that Tween-80 enhances the extraction of secondary metabolites more effectively than Triton X-100. High-Performance Liquid Chromatography (HPLC) analysis validated these outcomes. In conclusion, this research significantly contributes to optimizing plant tissue culture protocols for Aegle marmelos, advancing scientific understanding, and offering practical insights for improving the reproducibility and efficiency of Aegle marmelos cell cultures. These findings hold promise for potential publication, marking notable advancements in plant tissue culture and permeabilization strategies.