IN-VITRO HEPATOPROTECTIVE ACTIVITY OF METHANOLIC LEAF EXTRACT OF MURRAYA KOENIGII AGAINST CCL4 INDUCED HEPATOTOXICITY IN GOAT LIVER SLICE CULTURE

The purpose of the current investigation was to evaluate the hepatoprotective activity of methanolic leaf extract of Murraya koenigii in goat liver slice culture against carbon tetrachloride (CCl₄)-induced hepatotoxicity. The amount of cell damage caused by CCl₄ was measured by the release of marker enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH). Additionally, we estimated the levels of lipid peroxidation (LPO) in the membrane lipids of hepatocytes to assess the extent of hepatic injury. In comparison to untreated liver cells, those treated with CCl₄ resulted in a twofold increase in LPO, as well as the release of ALP, ALT, AST, ACP, and LDH at rates of  2.59, 3.34, 3.36, 2.85, and 6.75 times, respectively. Furthermore, liver cells treated with both CCl₄ and with methanolic leaf extract of Murraya koenigii exhibited significant reduction in hepatic marker enzymes. The 2,2-diphenylpicrylhydrazyl (DPPH) method was used to figure out the plant extract’s free radical scavenging activity (IC₅₀ 137±1.9 μg/mL). Qualitative analysis of the methanolic leaf extract revealed the presence of flavonoids (silymarin and quercetin). These findings suggest that the liver cells were shielded from CCl₄-induced oxidative/free radical-mediated injury in-vitro by the methanolic leaf extract of Murraya koenigii, due to the presence of flavonoids like silymarin and quercetin, which possess remarkable antioxidant properties providing hepatoprotective activity.