IN-VITRO AND IN-SILICO STUDY OF BUTEA MONOSPERMA FLOWER

The active compounds present in plants gained attention for their biological activity and this makes them important for the pharmaceutical industries. Butea monosperma has significant role in traditional medicines. Its leaves, bark, and flower have an important role against diabetes, filariasis, ulcers, inflammations, etc. However, till date no detailed phytochemicals profiling in flowers of B. monosperma along with their antioxidant and antidiabetic potential has reported. This study aims to measure antioxidant activity, total phenols, flavonoid contents in methanol and ethanol extract of the flower, and to identify their compounds through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Biological activity was determined by available literature present on NCBI, PubChem. However, Antioxidant actions were measured by DPPH and FRAP assays. Further antidiabetic potential of compound extract was analyzed using in-silico approach. It was found that methanolic extract showed better antioxidant potential than ethanolic extract. Total Phenol and flavonoid content were investigated but no significant difference was observed between the extracts. 42 major compounds were identified in methanolic extract using GC-MS, among which, 30 have well-reported structures and biological activities. D-Pinitol, present in methanolic extract at higher concentration were used for docking analysis to confirm their anti-diabetic properties by inhibiting the alpha amylase activity. This is the first report on antidiabetic potential of methanol extract of B. monosperma using in-silico approach. The results provide valuable data on the phytocompounds and their biological potential that can be further used for the treatment of other diseaseswhere oxidation and inflammation play a significant role.