ASSESSMENT OF ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED USING AQUEOUS EXTRACT OF SCHIZOPHYLLUM COMMUNE IN TRIAZOPHOS-INDUCED OXIDATIVE STRESS IN ALBINO RATS

The present work includes the green synthesis of silver nanoparticles using aqueous extract of edible macrofungus Schizophyllum commune, and to compare the antioxidant potentialities of the aqueous extract and the extract-based silver nanoparticles using Albino Wistar Rats. The synthesized silver nanoparticles showed the SPR (Surface Plasmon Resonance) peak at 431 in the UV-VIS spectrum. The SEM images showed that the nanoparticles have cuboidal and roughly spherical morphology with a size ranging from 80 nm to 110 nm with an average size of 96.13nm. The FT-IR spectrum revealed the surface coating of functional groups of different secondary metabolites present in the macrofungal extract. The results of in-vitro assessment of antioxidant activity revealed that the aqueous extract has 21.28 % and the extract-based silver nanoparticles have 33.59% DPPH radical scavenging activity at 100µg/ml concentration. The in-vivo analysis involved the assessment of activity of key enzymes like Catalase (CAT), Super Oxide Dismutase (SOD) and Glutathione Peroxidase (GPx) in albino rats and the results showed that in Triazophos-intoxicated rats the CAT and GPx activity significantly (p=0.05) increased and activity of SOD was significantly (p=0.05) decreased in comparison to the control group of rats. The rats treated with aqueous extract of S. commune extract and extract based silver nanoparticles showed a significant (p=0.05) decrease in the CAT and GPx activity and a significant (p=0.05) increase in SOD activity in comparison to the Triazophos-intoxicated rats showing significant antioxidant activities of both the extract and the extract-based silver nanoparticles.